Identification and characterization of a secreted M28 aminopeptidase protein in Acanthamoeba

Research output: Contribution to journalArticle

Abstract

Acanthamoeba is a free-living pathogenic protozoan that is distributed in different environmental reservoirs, including lakes and soil. Pathogenic Acanthamoeba can cause severe human diseases, such as blinding keratitis and granulomatous encephalitis. Therefore, it is important to understand the pathogenic relationship between humans and Acanthamoeba. By comparison of systemic analysis results for Acanthamoeba isolates, we identified a novel secreted protein of Acanthamoeba, an M28 aminopeptidase (M28AP), which targets of the human innate immune defense. We investigated the molecular functions and characteristics of the M28AP protein by anti-M28 antibodies and a M28AP mutant strain generated by the CRISPR/Cas9 system. Human complement proteins such as C3b and iC3b were degraded by Acanthamoeba M28AP. We believe that M28AP is an important factor in human innate immunity. This study provides new insight for the development of more efficient medicines to treat Acanthamoeba infection.

Original languageEnglish
Pages (from-to)1865-1874
Number of pages10
JournalParasitology Research
Volume118
Issue number6
DOIs
Publication statusPublished - 2019 Jun 1

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Acanthamoeba
Aminopeptidases
aminopeptidases
Proteins
proteins
Clustered Regularly Interspaced Short Palindromic Repeats
Complement C3b
keratitis
Keratitis
Encephalitis
encephalitis
Lakes
human diseases
Innate Immunity
Protozoa
Anti-Idiotypic Antibodies
Complement System Proteins
medicine
complement
Soil

All Science Journal Classification (ASJC) codes

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

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abstract = "Acanthamoeba is a free-living pathogenic protozoan that is distributed in different environmental reservoirs, including lakes and soil. Pathogenic Acanthamoeba can cause severe human diseases, such as blinding keratitis and granulomatous encephalitis. Therefore, it is important to understand the pathogenic relationship between humans and Acanthamoeba. By comparison of systemic analysis results for Acanthamoeba isolates, we identified a novel secreted protein of Acanthamoeba, an M28 aminopeptidase (M28AP), which targets of the human innate immune defense. We investigated the molecular functions and characteristics of the M28AP protein by anti-M28 antibodies and a M28AP mutant strain generated by the CRISPR/Cas9 system. Human complement proteins such as C3b and iC3b were degraded by Acanthamoeba M28AP. We believe that M28AP is an important factor in human innate immunity. This study provides new insight for the development of more efficient medicines to treat Acanthamoeba infection.",
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Identification and characterization of a secreted M28 aminopeptidase protein in Acanthamoeba. / Huang, Jian Ming; Chang, Yao Tsung; Shih, Min Hsiu; Lin, Wei Chen; Huang, Fu Chin.

In: Parasitology Research, Vol. 118, No. 6, 01.06.2019, p. 1865-1874.

Research output: Contribution to journalArticle

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