Identification and characterization of DSCAM isoforms isolated from orange-spotted grouper Epinephelus coioides

Ying Chun Yeh, Chung Wei Lee, Yi Wun Pan, Yi Jiou Hsu, Hsin Yi Hung, Yi-Min Chen, Han You Lin, Tzong-Yueh Chen, Huey Lang Yang, Han-Ching Wang

Research output: Contribution to journalArticle

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Abstract

The Down syndrome cell adhesion molecule (DSCAM), an immunoglobulin (Ig) superfamily member, was first identified from human and subsequently isolated from both vertebrates and invertebrates. Recent studies have shown that the DSCAM molecule serves diverse functions in neurodevelopment, such as axon guidance and neuronal migration. Most studies on DSCAM, however, have focused on mammals and arthropods, and our present knowledge of bony fish DSCAM is still limited. In this study, orange-spotted grouper . Epinephelus coioides was used as an animal model to explore the possible functions of DSCAM. Two DSCAM isoforms were isolated, namely EcDSCAM A and EcDSCAM B, with lengths of 1648 and 2025 amino acids, respectively. The classical domain structure (i.e. 9Ig-4FNIII-1Ig-2FNIII-Transmembrane domain-Cytoplasmic tail) was also found in the coding regions of these two EcDSCAMs. Phylogenetic analysis showed that in the vertebrate DSCAM clade, the EcDSCAMs and various teleost DSCAMs were clustered into a subclade. Real-time PCR revealed that EcDSCAM B is the major EcDSCAM isoform, with the expression of EcDSCAM B being significantly higher than that of EcDSCAM A. During the first 14. days after hatching (dph), increases in the expression of the two EcDSCAMs were observed at 2-4 and 8-11. dph. EcDSCAM is expressed mainly in the intestine, nerve-related tissues, and stomach. Optic nerve transection analysis showed that EcDSCAM was up-regulated during optic nerve regeneration after optic nerve injury. We also investigated whether DSCAM expression was affected by viral nervous necrosis (VNN) disease or vibriosis. We found that when grouper were challenged with nervous necrosis virus (NNV), there were no meaningful changes in DSCAM expression, but challenge with . Vibrio anguillarum led to a decrease in EcDSCAM levels in the brain. This decrease may be related to the pathogenesis of . V. anguillarum.

Original languageEnglish
Pages (from-to)148-159
Number of pages12
JournalDevelopmental and Comparative Immunology
Volume38
Issue number1
DOIs
Publication statusPublished - 2012 Sep 1

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Cell Adhesion Molecules
Down Syndrome
Protein Isoforms
Optic Nerve Injuries
Vertebrates
Necrosis
Nerve Tissue
Nerve Regeneration
Vibrio
Arthropods
Invertebrates
Optic Nerve
Intestines
Tail
Immunoglobulins
Real-Time Polymerase Chain Reaction
Mammals
Stomach
Fishes
Animal Models

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

Yeh, Ying Chun ; Lee, Chung Wei ; Pan, Yi Wun ; Hsu, Yi Jiou ; Hung, Hsin Yi ; Chen, Yi-Min ; Lin, Han You ; Chen, Tzong-Yueh ; Yang, Huey Lang ; Wang, Han-Ching. / Identification and characterization of DSCAM isoforms isolated from orange-spotted grouper Epinephelus coioides. In: Developmental and Comparative Immunology. 2012 ; Vol. 38, No. 1. pp. 148-159.
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abstract = "The Down syndrome cell adhesion molecule (DSCAM), an immunoglobulin (Ig) superfamily member, was first identified from human and subsequently isolated from both vertebrates and invertebrates. Recent studies have shown that the DSCAM molecule serves diverse functions in neurodevelopment, such as axon guidance and neuronal migration. Most studies on DSCAM, however, have focused on mammals and arthropods, and our present knowledge of bony fish DSCAM is still limited. In this study, orange-spotted grouper . Epinephelus coioides was used as an animal model to explore the possible functions of DSCAM. Two DSCAM isoforms were isolated, namely EcDSCAM A and EcDSCAM B, with lengths of 1648 and 2025 amino acids, respectively. The classical domain structure (i.e. 9Ig-4FNIII-1Ig-2FNIII-Transmembrane domain-Cytoplasmic tail) was also found in the coding regions of these two EcDSCAMs. Phylogenetic analysis showed that in the vertebrate DSCAM clade, the EcDSCAMs and various teleost DSCAMs were clustered into a subclade. Real-time PCR revealed that EcDSCAM B is the major EcDSCAM isoform, with the expression of EcDSCAM B being significantly higher than that of EcDSCAM A. During the first 14. days after hatching (dph), increases in the expression of the two EcDSCAMs were observed at 2-4 and 8-11. dph. EcDSCAM is expressed mainly in the intestine, nerve-related tissues, and stomach. Optic nerve transection analysis showed that EcDSCAM was up-regulated during optic nerve regeneration after optic nerve injury. We also investigated whether DSCAM expression was affected by viral nervous necrosis (VNN) disease or vibriosis. We found that when grouper were challenged with nervous necrosis virus (NNV), there were no meaningful changes in DSCAM expression, but challenge with . Vibrio anguillarum led to a decrease in EcDSCAM levels in the brain. This decrease may be related to the pathogenesis of . V. anguillarum.",
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Identification and characterization of DSCAM isoforms isolated from orange-spotted grouper Epinephelus coioides. / Yeh, Ying Chun; Lee, Chung Wei; Pan, Yi Wun; Hsu, Yi Jiou; Hung, Hsin Yi; Chen, Yi-Min; Lin, Han You; Chen, Tzong-Yueh; Yang, Huey Lang; Wang, Han-Ching.

In: Developmental and Comparative Immunology, Vol. 38, No. 1, 01.09.2012, p. 148-159.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification and characterization of DSCAM isoforms isolated from orange-spotted grouper Epinephelus coioides

AU - Yeh, Ying Chun

AU - Lee, Chung Wei

AU - Pan, Yi Wun

AU - Hsu, Yi Jiou

AU - Hung, Hsin Yi

AU - Chen, Yi-Min

AU - Lin, Han You

AU - Chen, Tzong-Yueh

AU - Yang, Huey Lang

AU - Wang, Han-Ching

PY - 2012/9/1

Y1 - 2012/9/1

N2 - The Down syndrome cell adhesion molecule (DSCAM), an immunoglobulin (Ig) superfamily member, was first identified from human and subsequently isolated from both vertebrates and invertebrates. Recent studies have shown that the DSCAM molecule serves diverse functions in neurodevelopment, such as axon guidance and neuronal migration. Most studies on DSCAM, however, have focused on mammals and arthropods, and our present knowledge of bony fish DSCAM is still limited. In this study, orange-spotted grouper . Epinephelus coioides was used as an animal model to explore the possible functions of DSCAM. Two DSCAM isoforms were isolated, namely EcDSCAM A and EcDSCAM B, with lengths of 1648 and 2025 amino acids, respectively. The classical domain structure (i.e. 9Ig-4FNIII-1Ig-2FNIII-Transmembrane domain-Cytoplasmic tail) was also found in the coding regions of these two EcDSCAMs. Phylogenetic analysis showed that in the vertebrate DSCAM clade, the EcDSCAMs and various teleost DSCAMs were clustered into a subclade. Real-time PCR revealed that EcDSCAM B is the major EcDSCAM isoform, with the expression of EcDSCAM B being significantly higher than that of EcDSCAM A. During the first 14. days after hatching (dph), increases in the expression of the two EcDSCAMs were observed at 2-4 and 8-11. dph. EcDSCAM is expressed mainly in the intestine, nerve-related tissues, and stomach. Optic nerve transection analysis showed that EcDSCAM was up-regulated during optic nerve regeneration after optic nerve injury. We also investigated whether DSCAM expression was affected by viral nervous necrosis (VNN) disease or vibriosis. We found that when grouper were challenged with nervous necrosis virus (NNV), there were no meaningful changes in DSCAM expression, but challenge with . Vibrio anguillarum led to a decrease in EcDSCAM levels in the brain. This decrease may be related to the pathogenesis of . V. anguillarum.

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