Identification and discrimination of bacteria using Fourier transform infrared spectroscopy

Jyoti Prakash Maity, Sandeep Kar, Chao Ming Lin, Chen Yen Chen, Young Fo Chang, Jiin Shuh Jean, Thomas R. Kulp

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

(Graph Presented) Bacterial spectra were obtained in the wavenumber range of 4000-600 cm-1 using FTIR spectroscopy. FTIR spectral patterns were analyzed and matched with 16S-rRNA signatures of bacterial strains OS 1 and OS2, isolated from oil sludge. Specific spectral bands obtained from OS1 (FJ226761), reference strainBacillus flexus (ATCC 49095), OS2 (FJ215874) and reference strain Stenotrophomonas maltophilia (ATCC 19861) respectively, suggested that OS1 and ATCC 49095 were closely related whereas OS2 was different. The bands probably represent groups of proteins and lipids of specific bacteria. Separate peaks found in B. flexus were similar to those of OS1. The S. maltophilia (ATCC 19861) and OS2 exhibited a similar peak at 3272 cm -1. Amide bands (I, II and III) exhibited that OS1 and B. flexus were closely related, but were different from OS2. In the fingerprint region, peak at 1096 cm-1 and 1360 cm-1 exhibited the specific fingerprints of OS2 and reference strain S. maltophilia (ATCC 19861), respectively. The specific fingerprint signature was found at 1339 cm-1 for OS1 and at 1382 cm-1 for B. flexus ATCC 49095, allowing these two strains of B. flexus to be differentiated. This spectral signature originated from phospholipid and RNA components of the cell. Principle components analysis (PCA) of spectral regions exhibited with distinct sample clusters between Bacillus flexus (ATCC 49095), S. maltophilia (ATCC 19861), OS1 and OS2 in amide and fingerprint region.

Original languageEnglish
Pages (from-to)478-484
Number of pages7
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume116
DOIs
Publication statusPublished - 2013

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

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