@article{3876c742b8d24444b2ee5c337db14149,
title = "Identification of a gut microbiota member that ameliorates DSS-induced colitis in intestinal barrier enhanced Dusp6-deficient mice",
abstract = "Strengthening the gut epithelial barrier is a potential strategy for management of gut microbiota-associated illnesses. Here, we demonstrate that dual-specificity phosphatase 6 (Dusp6) knockout enhances baseline colon barrier integrity and ameliorates dextran sulfate sodium (DSS)-induced colonic injury. DUSP6 mutation in Caco-2 cells enhances the epithelial feature and increases mitochondrial oxygen consumption, accompanied by altered glucose metabolism and decreased glycolysis. We find that Dusp6-knockout mice are more resistant to DSS-induced dysbiosis, and the cohousing and fecal microbiota transplantation experiments show that the gut/fecal microbiota derived from Dusp6-knockout mice also confers protection against colitis. Further culturomics and mono-colonialization experiments show that one gut microbiota member in the genus Duncaniella confers host protection from DSS-induced injury. We identify Dusp6 deficiency as beneficial for shaping the gut microbiota eubiosis necessary to protect against gut barrier-related diseases.",
author = "Chang, {Cherng Shyang} and Liao, {Yi Chu} and Huang, {Chih Ting} and Lin, {Chiao Mei} and Cheung, {Chantal Hoi Yin} and Ruan, {Jhen Wei} and Yu, {Wen Hsuan} and Tsai, {Yi Ting} and Lin, {I. Jung} and Huang, {Chien Hsun} and Liou, {Jong Shian} and Chou, {Ya Hsien} and Chien, {Hung Jen} and Chuang, {Hsiao Li} and Juan, {Hsueh Fen} and Huang, {Hsuan Cheng} and Chan, {Hong Lin} and Liao, {Yu Chieh} and Tang, {Shiue Cheng} and Su, {Yu Wen} and Tan, {Tse Hua} and B{\"a}umler, {Andreas J.} and Kao, {Cheng Yuan}",
note = "Funding Information: We thank Wann-Neng Jane (Cell Biology Core Lab, Academia Sinica) for transmission electron microscopy, Shau-Ku Huang (National Institute of Environmental Health Sciences, National Health Research Institutes [NHRI]) for critical and stimulating discussions during the preparation of the manuscript, Deng-Yu Jiang for assistance with western blotting, and Loretta Collins (WriteScience, LLC) for scientific editing and proofreading. We are grateful to the National Center for High-Performance Computing (NARLabs) for the use of computers and facilities, to the National Laboratory Animal Center (NARLabs) for technical support in service of isolators, and to the confocal imaging core in National Tsing Hua University (sponsored by MOST , Taiwan grant 108-2731-M-007-001- ) for imaging support. This work was supported by the NHRI , Taiwan ( IM-108-PP-04 ) and MOST , Taiwan ( 106-2628-B-400-001-MY3 , 106-2923-B-400-001-MY3 , 108-2321-B-400-011- , 109-2327-B-400-001- , 109-2320-B-002-017-MY3 , 109-2221-E-010-012-MY3 , 109-2320-B-400-008-MY3 , and 110-2327-B-400-005- ). The funding agencies had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: We thank Wann-Neng Jane (Cell Biology Core Lab, Academia Sinica) for transmission electron microscopy, Shau-Ku Huang (National Institute of Environmental Health Sciences, National Health Research Institutes [NHRI]) for critical and stimulating discussions during the preparation of the manuscript, Deng-Yu Jiang for assistance with western blotting, and Loretta Collins (WriteScience, LLC) for scientific editing and proofreading. We are grateful to the National Center for High-Performance Computing (NARLabs) for the use of computers and facilities, to the National Laboratory Animal Center (NARLabs) for technical support in service of isolators, and to the confocal imaging core in National Tsing Hua University (sponsored by MOST, Taiwan grant 108-2731-M-007-001-) for imaging support. This work was supported by the NHRI, Taiwan (IM-108-PP-04) and MOST, Taiwan (106-2628-B-400-001-MY3, 106-2923-B-400-001-MY3, 108-2321-B-400-011-, 109-2327-B-400-001-, 109-2320-B-002-017-MY3, 109-2221-E-010-012-MY3, 109-2320-B-400-008-MY3, and 110-2327-B-400-005-). The funding agencies had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. C.-S.C. H.-F.J. H.-C.H. H.-L. Chan, Yi-Chu Liao, Yu-Chieh Liao, S.-C.T. A.J.B. and C.-Y.K. designed the study and interpreted data. C.-S.C. C.H.Y.C. C.-T.H. C.-M.L. J.-W.R. H.-L. Chuang, and Y.-T.T. performed experiments and data analyses. Yi-Chu Liao, Yu-Chieh Liao, W.-H.Y. I.-J.L. and C.-Y.K. performed bioinformatic analyses. J.-S.L. and C.-H.H. performed culturomics analysis. Y.-H.C. and H.-J.C. performed 3D visualization. T.-H.T. and Y.-W.S. provided D6KO mice. C.-S.C. and C.-Y.K. wrote the manuscript. C.-Y.K. supervised the project. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = nov,
day = "23",
doi = "10.1016/j.celrep.2021.110016",
language = "English",
volume = "37",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "8",
}