TY - JOUR
T1 - Identification of a rod domain-truncated isoform of nestin, Nes-Sδ107-254, in rat dorsal root ganglia
AU - Wong, Zong Ruei
AU - Su, Peng Han
AU - Chang, Kai Wei
AU - Huang, Bu Miin
AU - Lee, Hsinyu
AU - Yang, Hsi Yuan
N1 - Funding Information:
We thank the Technology Commons, College of Life Science, National Taiwan University , for their microscopy equipment. This work was supported by the National Science Council, Executive Yuan, Taiwan through grants to H.-Y.Y. (NSC101-2311-B-002-003-) and B.-M.H. (NSC101-2320-B-006-005-MY3).
PY - 2013/10/11
Y1 - 2013/10/11
N2 - Nestin, a type VI intermediate filament (IF) protein, is predominantly expressed in neurogenic and myogenic stem cells. We previously identified the first isoform of nestin, Nes-S, in rat DRG neurons. In this study, we report a previously unidentified nestin isoform, Nes-Sδ107-254, that is expressed in lower levels in DRG neurons of adult rats. The 29-kD Nes-Sδ107-254 is identical to Nes-S, except that an additional region is removed in its rod domain. Nes-Sδ107-254 is assembly compromised and forms aggregates. Unlike nestin and Nes-S, Nes-Sδ107-254 does not exert cytoprotective effect. Furthermore, elevated caspase-3 activation was observed in HEK293T cells expressing the EGFP-Nes-Sδ107-254 protein. Taken together, Nes-Sδ107-254 is a rod domain-truncated isoform of nestin that is susceptible to form cytotoxic aggregates.
AB - Nestin, a type VI intermediate filament (IF) protein, is predominantly expressed in neurogenic and myogenic stem cells. We previously identified the first isoform of nestin, Nes-S, in rat DRG neurons. In this study, we report a previously unidentified nestin isoform, Nes-Sδ107-254, that is expressed in lower levels in DRG neurons of adult rats. The 29-kD Nes-Sδ107-254 is identical to Nes-S, except that an additional region is removed in its rod domain. Nes-Sδ107-254 is assembly compromised and forms aggregates. Unlike nestin and Nes-S, Nes-Sδ107-254 does not exert cytoprotective effect. Furthermore, elevated caspase-3 activation was observed in HEK293T cells expressing the EGFP-Nes-Sδ107-254 protein. Taken together, Nes-Sδ107-254 is a rod domain-truncated isoform of nestin that is susceptible to form cytotoxic aggregates.
UR - http://www.scopus.com/inward/record.url?scp=84884399084&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884399084&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2013.08.035
DO - 10.1016/j.neulet.2013.08.035
M3 - Article
C2 - 23994057
AN - SCOPUS:84884399084
SN - 0304-3940
VL - 553
SP - 181
EP - 185
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -