TY - JOUR
T1 - Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property using supercritical carbon dioxide extraction
AU - Wang, Hui Min
AU - Pan, Jian Liang
AU - Chen, Chun Yen
AU - Chiu, Chien Chich
AU - Yang, Mu Hoe
AU - Chang, Hsueh Wei
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial supports from Taiwan's National Science Council under grant numbers: NSC 95-2221-E-006-164-MY3 , NSC 96-2628-E-006-004-MY3 , NSC 96-2218-E-006-295 , NSC 97-2218-E-006-005 , NSC97-2221-E-037-002- and NSC98-2221-E-037-005- , from Kaoshiung Medical University (grant no. KMU-EM-99-1.4 ), and from Ministry of Economic Affairs (grant no. 98-EC-17-A-10-S2-0066 ). The authors also would like to thank Min-Yii Julie Wang for English editing and Ya-Ling Yeh, Jung-Haung Yan and Chi-Chen Yeh for the assistances.
PY - 2010/12
Y1 - 2010/12
N2 - In this study, supercritical carbon dioxide extraction (SC-CO2) technology was developed to extract the active components (such as antioxidants) from a novel microalga, Chlorella vulgaris C-C, due to its superior advantages over conventional solvent or ultrasonic extraction methods. Using SC-CO2 and ultrasonic extractions, the polyphenol contents of C. vulgaris C-C were 13.40 and 0.46 (mggallic acid/g lyophilized extract), respectively. The flavonoid content obtained from SC-CO2 (3.18mgquercetin/g lyophilized extract) was also significantly higher than from ultrasonic extraction (0.86mgquercetin/g lyophilized extract). Investigation of C. vulgaris C-C extract from SC-CO2 indicates strong antioxidant activities in radical scavenging, ferric reducing power and metal chelating abilities. In cell proliferation assay, the extract of C. vulgaris C-C inhibits human lung cancer H1299, A549, and H1437 cells in a dose-dependent manner. In addition, the treatment with extracts of C. vulgaris C-C effectively reduced the migration of tumor cells, suggesting the potential of using the C. vulgaris C-C extract to inhibit lung cancer metastasis.
AB - In this study, supercritical carbon dioxide extraction (SC-CO2) technology was developed to extract the active components (such as antioxidants) from a novel microalga, Chlorella vulgaris C-C, due to its superior advantages over conventional solvent or ultrasonic extraction methods. Using SC-CO2 and ultrasonic extractions, the polyphenol contents of C. vulgaris C-C were 13.40 and 0.46 (mggallic acid/g lyophilized extract), respectively. The flavonoid content obtained from SC-CO2 (3.18mgquercetin/g lyophilized extract) was also significantly higher than from ultrasonic extraction (0.86mgquercetin/g lyophilized extract). Investigation of C. vulgaris C-C extract from SC-CO2 indicates strong antioxidant activities in radical scavenging, ferric reducing power and metal chelating abilities. In cell proliferation assay, the extract of C. vulgaris C-C inhibits human lung cancer H1299, A549, and H1437 cells in a dose-dependent manner. In addition, the treatment with extracts of C. vulgaris C-C effectively reduced the migration of tumor cells, suggesting the potential of using the C. vulgaris C-C extract to inhibit lung cancer metastasis.
UR - http://www.scopus.com/inward/record.url?scp=78149496063&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78149496063&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2010.05.023
DO - 10.1016/j.procbio.2010.05.023
M3 - Article
AN - SCOPUS:78149496063
SN - 1359-5113
VL - 45
SP - 1865
EP - 1872
JO - Process Biochemistry
JF - Process Biochemistry
IS - 12
ER -
