Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property using supercritical carbon dioxide extraction

Hui Min Wang, Jian Liang Pan, Chun-Yen Chen, Chien Chich Chiu, Mu Hoe Yang, Hsueh Wei Chang, Jo-Shu Chang

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

In this study, supercritical carbon dioxide extraction (SC-CO2) technology was developed to extract the active components (such as antioxidants) from a novel microalga, Chlorella vulgaris C-C, due to its superior advantages over conventional solvent or ultrasonic extraction methods. Using SC-CO2 and ultrasonic extractions, the polyphenol contents of C. vulgaris C-C were 13.40 and 0.46 (mggallic acid/g lyophilized extract), respectively. The flavonoid content obtained from SC-CO2 (3.18mgquercetin/g lyophilized extract) was also significantly higher than from ultrasonic extraction (0.86mgquercetin/g lyophilized extract). Investigation of C. vulgaris C-C extract from SC-CO2 indicates strong antioxidant activities in radical scavenging, ferric reducing power and metal chelating abilities. In cell proliferation assay, the extract of C. vulgaris C-C inhibits human lung cancer H1299, A549, and H1437 cells in a dose-dependent manner. In addition, the treatment with extracts of C. vulgaris C-C effectively reduced the migration of tumor cells, suggesting the potential of using the C. vulgaris C-C extract to inhibit lung cancer metastasis.

Original languageEnglish
Pages (from-to)1865-1872
Number of pages8
JournalProcess Biochemistry
Volume45
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

Chlorella vulgaris
Antioxidants
Carbon Dioxide
Lung Neoplasms
Carbon dioxide
Ultrasonics
Flavonoids
Scavenging
Cell proliferation
Polyphenols
Chelation
Tumors
Assays
Metals
Cells
Acids
Cell Movement
Cell Proliferation
Neoplasm Metastasis
Technology

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Wang, Hui Min ; Pan, Jian Liang ; Chen, Chun-Yen ; Chiu, Chien Chich ; Yang, Mu Hoe ; Chang, Hsueh Wei ; Chang, Jo-Shu. / Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property using supercritical carbon dioxide extraction. In: Process Biochemistry. 2010 ; Vol. 45, No. 12. pp. 1865-1872.
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Identification of anti-lung cancer extract from Chlorella vulgaris C-C by antioxidant property using supercritical carbon dioxide extraction. / Wang, Hui Min; Pan, Jian Liang; Chen, Chun-Yen; Chiu, Chien Chich; Yang, Mu Hoe; Chang, Hsueh Wei; Chang, Jo-Shu.

In: Process Biochemistry, Vol. 45, No. 12, 01.12.2010, p. 1865-1872.

Research output: Contribution to journalArticle

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