Identification of colorectal cancer recurrence-related microRNAs

Jian an Ju, Yuan ching Huang, Sheng hui Lan, Ting huei Wang, Peng-Chan Lin, Jheng chang Lee, Ko chi Niu, Yu feng Tian, Hsiao-Sheng Liu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Colorectal cancer (CRC) has high recurrence rate. Effective biomarkers for the detection of colon cancer are still unavailable. MicroRNA (miRNA) is an epigenetic factor that regulates cell proliferation, tumor cell growth, cancer formation, and metastasis by regulating tumor suppressor genes or oncogenes. miRNA has the potential to be used as a biomarker for the diagnosis of diverse cancers. Previously, we revealed that miR-139-3 p is downregulated and miR-338-5 p is upregulated in recurrent CRC patients. The present study further reveals that the miR-139-3 p expression level is inversely correlated with increased metastatic status in three colon cancer cell lines (SW480, SW620, and colo201), as determined using real-time polymerase chain reaction. Furthermore, in 51 pairs of CRC clinical specimens, the expression level of mir-139-3 p was significantly lower in the tumor sections than in the adjacent normal tissues (p < 0.0001), indicating that they are tumor-suppressive miRNAs. Furthermore, the expression level of mir-338-5 p in the tumor tissues of metastatic patients was significantly higher than in the tumor tissues of nonmetastatic patients (p < 0.05), indicating that mir-338-5 p is positively correlated with metastasis. All together, mir-139-3 p and mir-338-5 p may have potential use as biomarkers for the diagnosis of tumor formation and metastasis in CRC patients.

Original languageEnglish
Pages (from-to)19-20
Number of pages2
JournalGenomic Medicine, Biomarkers, and Health Sciences
Volume4
Issue number1-2
DOIs
Publication statusPublished - 2012 Mar 1

Fingerprint

MicroRNAs
Tumors
Colorectal Neoplasms
Recurrence
Biomarkers
Neoplasms
Tissue
Neoplasm Metastasis
Colonic Neoplasms
Polymerase chain reaction
Cell proliferation
Cell growth
Tumor Biomarkers
Tumor Suppressor Genes
Oncogenes
Epigenomics
Genes
Real-Time Polymerase Chain Reaction
Cells
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology(all)
  • Drug Discovery

Cite this

Ju, Jian an ; Huang, Yuan ching ; Lan, Sheng hui ; Wang, Ting huei ; Lin, Peng-Chan ; Lee, Jheng chang ; Niu, Ko chi ; Tian, Yu feng ; Liu, Hsiao-Sheng. / Identification of colorectal cancer recurrence-related microRNAs. In: Genomic Medicine, Biomarkers, and Health Sciences. 2012 ; Vol. 4, No. 1-2. pp. 19-20.
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abstract = "Colorectal cancer (CRC) has high recurrence rate. Effective biomarkers for the detection of colon cancer are still unavailable. MicroRNA (miRNA) is an epigenetic factor that regulates cell proliferation, tumor cell growth, cancer formation, and metastasis by regulating tumor suppressor genes or oncogenes. miRNA has the potential to be used as a biomarker for the diagnosis of diverse cancers. Previously, we revealed that miR-139-3 p is downregulated and miR-338-5 p is upregulated in recurrent CRC patients. The present study further reveals that the miR-139-3 p expression level is inversely correlated with increased metastatic status in three colon cancer cell lines (SW480, SW620, and colo201), as determined using real-time polymerase chain reaction. Furthermore, in 51 pairs of CRC clinical specimens, the expression level of mir-139-3 p was significantly lower in the tumor sections than in the adjacent normal tissues (p < 0.0001), indicating that they are tumor-suppressive miRNAs. Furthermore, the expression level of mir-338-5 p in the tumor tissues of metastatic patients was significantly higher than in the tumor tissues of nonmetastatic patients (p < 0.05), indicating that mir-338-5 p is positively correlated with metastasis. All together, mir-139-3 p and mir-338-5 p may have potential use as biomarkers for the diagnosis of tumor formation and metastasis in CRC patients.",
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Ju, JA, Huang, YC, Lan, SH, Wang, TH, Lin, P-C, Lee, JC, Niu, KC, Tian, YF & Liu, H-S 2012, 'Identification of colorectal cancer recurrence-related microRNAs', Genomic Medicine, Biomarkers, and Health Sciences, vol. 4, no. 1-2, pp. 19-20. https://doi.org/10.1016/j.gmbhs.2012.04.005

Identification of colorectal cancer recurrence-related microRNAs. / Ju, Jian an; Huang, Yuan ching; Lan, Sheng hui; Wang, Ting huei; Lin, Peng-Chan; Lee, Jheng chang; Niu, Ko chi; Tian, Yu feng; Liu, Hsiao-Sheng.

In: Genomic Medicine, Biomarkers, and Health Sciences, Vol. 4, No. 1-2, 01.03.2012, p. 19-20.

Research output: Contribution to journalArticle

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AU - Ju, Jian an

AU - Huang, Yuan ching

AU - Lan, Sheng hui

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AU - Tian, Yu feng

AU - Liu, Hsiao-Sheng

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