Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

Chi Jung Wu; Ya Ping Chen; Hsuan Chen Wang; Ih Jen Su; Wen Chien Ko; Jiann Shiuh Chen; Chao Neng Cheng; Nan Yao Lee; H. Sunny Sun; Chia Yu Chi; Tsai Yun Chen

doi:10.1016/j.diagmicrobio.2013.08.007

Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

Chi Jung Wu, Ya Ping Chen, Hsuan Chen Wang, Ih Jen Su, Wen Chien Ko, Jiann Shiuh Chen, Chao Neng Cheng, Nan Yao Lee, H. Sunny Sun, Chia Yu Chi, Tsai Yun Chen

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.

Original language	English
Pages (from-to)	141-143
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	78
Issue number	2
DOIs	https://doi.org/10.1016/j.diagmicrobio.2013.08.007
Publication status	Published - 2014 Feb

All Science Journal Classification (ASJC) codes

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2013.08.007

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Wu, C. J., Chen, Y. P., Wang, H. C., Su, I. J., Ko, W. C., Chen, J. S., Cheng, C. N., Lee, N. Y., Sun, H. S., Chi, C. Y., & Chen, T. Y. (2014). Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry. Diagnostic Microbiology and Infectious Disease, 78(2), 141-143. https://doi.org/10.1016/j.diagmicrobio.2013.08.007

@article{01838d96a995484488a463bd1564063b,

title = "Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry",

abstract = "Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.",

author = "Wu, {Chi Jung} and Chen, {Ya Ping} and Wang, {Hsuan Chen} and Su, {Ih Jen} and Ko, {Wen Chien} and Chen, {Jiann Shiuh} and Cheng, {Chao Neng} and Lee, {Nan Yao} and Sun, {H. Sunny} and Chi, {Chia Yu} and Chen, {Tsai Yun}",

note = "Funding Information: This study was supported by grants from the National Health Research Institutes (IV-101-SP-18). We thank Miss Ming-I Hsieh for the laboratory work.",

year = "2014",

month = feb,

doi = "10.1016/j.diagmicrobio.2013.08.007",

language = "English",

volume = "78",

pages = "141--143",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier Inc.",

number = "2",

}

Wu, CJ, Chen, YP, Wang, HC, Su, IJ, Ko, WC , Chen, JS , Cheng, CN , Lee, NY , Sun, HS, Chi, CY & Chen, TY 2014, 'Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry', Diagnostic Microbiology and Infectious Disease, vol. 78, no. 2, pp. 141-143. https://doi.org/10.1016/j.diagmicrobio.2013.08.007

TY - JOUR

T1 - Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

AU - Wu, Chi Jung

AU - Chen, Ya Ping

AU - Wang, Hsuan Chen

AU - Su, Ih Jen

AU - Ko, Wen Chien

AU - Chen, Jiann Shiuh

AU - Cheng, Chao Neng

AU - Lee, Nan Yao

AU - Sun, H. Sunny

AU - Chi, Chia Yu

AU - Chen, Tsai Yun

N1 - Funding Information: This study was supported by grants from the National Health Research Institutes (IV-101-SP-18). We thank Miss Ming-I Hsieh for the laboratory work.

PY - 2014/2

Y1 - 2014/2

N2 - Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.

AB - Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.

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UR - http://www.scopus.com/inward/citedby.url?scp=84892482331&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2013.08.007

DO - 10.1016/j.diagmicrobio.2013.08.007

M3 - Article

C2 - 24268535

AN - SCOPUS:84892482331

SN - 0732-8893

VL - 78

SP - 141

EP - 143

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 2

ER -

Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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