Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

Chi Jung Wu, Ya-Ping Chen, Hsuan Chen Wang, Ih Jen Su, Wen-Chien Ko, Chien-Hsu Chen, Chao-Neng Cheng, Nan-Yao Lee, Hsiao-Fang Sun, Chia Yu Chi, Tsai-Yun Chen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.

Original languageEnglish
Pages (from-to)141-143
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume78
Issue number2
DOIs
Publication statusPublished - 2014 Feb 1

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Electrospray Ionization Mass Spectrometry
Polymerase Chain Reaction
Mycoses
Body Fluids
Fungi
Biopsy

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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abstract = "Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.",
author = "Wu, {Chi Jung} and Ya-Ping Chen and Wang, {Hsuan Chen} and Su, {Ih Jen} and Wen-Chien Ko and Chien-Hsu Chen and Chao-Neng Cheng and Nan-Yao Lee and Hsiao-Fang Sun and Chi, {Chia Yu} and Tsai-Yun Chen",
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AU - Wu, Chi Jung

AU - Chen, Ya-Ping

AU - Wang, Hsuan Chen

AU - Su, Ih Jen

AU - Ko, Wen-Chien

AU - Chen, Chien-Hsu

AU - Cheng, Chao-Neng

AU - Lee, Nan-Yao

AU - Sun, Hsiao-Fang

AU - Chi, Chia Yu

AU - Chen, Tsai-Yun

PY - 2014/2/1

Y1 - 2014/2/1

N2 - Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.

AB - Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed.

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