Identification of gold sensing peptide by integrative proteomics and a bacterial two-component system

I. Son Ng, You Jin Yu, Ying Chen Yi, Shih I. Tan, Bo Chuan Huang, Yin Lung Han

Research output: Contribution to journalArticle

Abstract

The proteomics strategy was utilized to analyze and identify the gold adsorption proteins from Tepidimonas fonticaldi AT-A2, due to its outstanding performance in gold-binding and recovery. The results showed that three small proteins, including histidine biosynthesis protein (HisIE), iron donor protein (CyaY) and hypothetical protein_65aa, have a higher ability to adsorb gold ions because of the negatively charged domains or metal binding sites. On the other hand, the Salmonella PmrA/PmrB two-component system first replaces the iron (III)-binding motif using the peptide sequence from hypothetical protein_65aa, and this is then used to reveal the sensing and responsiveness to gold metal ions, which is totally different from the performance of traditional gold binding peptide (GBP) on the crystals on the surface of gold (111). We have successfully demonstrated an integrative proteomics and bacterial two-component system to explore the novel GBP. Finally, the heterologous over-expression of GBP by E. coli and the equilibrium of binding capacity for Au(III) have been conducted.

Original languageEnglish
Article number127
JournalFrontiers in Chemistry
Volume5
Issue numberDEC
DOIs
Publication statusPublished - 2017 Jan 1

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Gold
Peptides
Proteins
Iron
Salmonella
Proteomics
Biosynthesis
Histidine
Escherichia coli
Metal ions
Metals
Binding Sites
Ions
Adsorption
Recovery
Crystals

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Ng, I. Son ; Yu, You Jin ; Yi, Ying Chen ; Tan, Shih I. ; Huang, Bo Chuan ; Han, Yin Lung. / Identification of gold sensing peptide by integrative proteomics and a bacterial two-component system. In: Frontiers in Chemistry. 2017 ; Vol. 5, No. DEC.
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Identification of gold sensing peptide by integrative proteomics and a bacterial two-component system. / Ng, I. Son; Yu, You Jin; Yi, Ying Chen; Tan, Shih I.; Huang, Bo Chuan; Han, Yin Lung.

In: Frontiers in Chemistry, Vol. 5, No. DEC, 127, 01.01.2017.

Research output: Contribution to journalArticle

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