Identification of human hepatocellular carcinoma-related proteins by proteomic approaches

Ming Hui Yang, Yu Chang Tyan, Shiang Bin Jong, Ying Fong Huang, Pao-Chi Liao, Ming Chen Wang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. Analysis of human serum from HCC patients using two-dimensional gel electrophoresis (2DE) combined with nano-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) identified fourteen different proteins differentially expressed between HCC patients and the control group. Twelve proteins were up-regulated and two down-regulated. By using nano-HPLC-MS/MS system to analyze proteome in human serum, 317 proteins were identified, twenty-nine of which to high confidence levels (protein matched at last two unique peptide sequences). Of these twenty-nine proteins, six were present only in HCC patients and may serve as biomarkers for HCC.

Original languageEnglish
Pages (from-to)637-643
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume388
Issue number3
DOIs
Publication statusPublished - 2007 Jun 1

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Forensic Anthropology
Proteomics
Hepatocellular Carcinoma
High Pressure Liquid Chromatography
Proteins
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
High performance liquid chromatography
Biomarkers
Proteome
Tandem Mass Spectrometry
Electrophoresis
Liver
Mass spectrometry
Blood Proteins
Tumors
Gels
Control Groups
Peptides

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Yang, Ming Hui ; Tyan, Yu Chang ; Jong, Shiang Bin ; Huang, Ying Fong ; Liao, Pao-Chi ; Wang, Ming Chen. / Identification of human hepatocellular carcinoma-related proteins by proteomic approaches. In: Analytical and Bioanalytical Chemistry. 2007 ; Vol. 388, No. 3. pp. 637-643.
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Identification of human hepatocellular carcinoma-related proteins by proteomic approaches. / Yang, Ming Hui; Tyan, Yu Chang; Jong, Shiang Bin; Huang, Ying Fong; Liao, Pao-Chi; Wang, Ming Chen.

In: Analytical and Bioanalytical Chemistry, Vol. 388, No. 3, 01.06.2007, p. 637-643.

Research output: Contribution to journalArticle

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