Identification of human hepatocellular carcinoma-related proteins by proteomic approaches

Ming Hui Yang, Yu Chang Tyan, Shiang Bin Jong, Ying Fong Huang, Pao Chi Liao, Ming Chen Wang

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)


Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. Analysis of human serum from HCC patients using two-dimensional gel electrophoresis (2DE) combined with nano-high-performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) identified fourteen different proteins differentially expressed between HCC patients and the control group. Twelve proteins were up-regulated and two down-regulated. By using nano-HPLC-MS/MS system to analyze proteome in human serum, 317 proteins were identified, twenty-nine of which to high confidence levels (protein matched at last two unique peptide sequences). Of these twenty-nine proteins, six were present only in HCC patients and may serve as biomarkers for HCC.

Original languageEnglish
Pages (from-to)637-643
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Issue number3
Publication statusPublished - 2007 Jun

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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