Identification of in vivo phosphorylation sites of CD45 protein-tyrosine phosphatase in 70Z/3.12 Cells

Sanmao Kang, Pao-Chi Liao, Douglas A. Gage, Walter J. Esselman

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Abstract

Phosphorylation of CD45, a transmembrane protein-tyrosine phosphatase (PTPase) has been proposed to mediate docking of signaling proteins and to modulate PTPase activity. To study the role of phosphorylation in CD45, in vivo phosphorylation sites of CD45 from 70Z/3.12 cells were identified using 32P labeling, trypsin digestion, two-dimensional peptide mapping, high performance liquid chromatography, phosphoamino acid analysis, matrix- assisted laser desorption/ionization mass spectrometry, and specific enzymatic degradation. Eight phosphopeptides, a through h, were isolated and four phosphorylation sites were identified. All four phosphorylation sites were in the membrane-distal PTPase domain (D2) and the C-terminal tail and none were in the membrane-proximal PTPase domain (D1). One site, Ser(P)939 peptide h, was in the D2 domain and, by comparison to the three-dimensional structure of PTP1B, is predicted to lie at the apex of the substrate binding loop. Ser938 was the only in vitro phosphorylation site for protein kinase C among the phosphorylation sites identified. Four of the C-terminal peptides identified (d, e, f, and g) spanned the same sequence and were derived from the same phosphorylation site in the C-terminal tail, Ser1204. Peptide a was derived from the intact C terminus and comprised a mixture of monophosphorylated peptides containing either Ser(P)1248 or Thr(P)1246. Knowledge of the precise phosphorylation sites of CD45 will lead to the design of experiments to define the role of phosphorylation in PTPase activity and in signaling.

Original languageEnglish
Pages (from-to)11588-11596
Number of pages9
JournalJournal of Biological Chemistry
Volume272
Issue number17
DOIs
Publication statusPublished - 1997 Apr 25

Fingerprint

Phosphorylation
Protein Tyrosine Phosphatases
Peptides
Membrane Proteins
Phosphoamino Acids
Membranes
Phosphopeptides
Peptide Mapping
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
High performance liquid chromatography
Design of experiments
Trypsin
Labeling
Protein Kinase C
Ionization
Mass spectrometry
Digestion
Desorption
High Pressure Liquid Chromatography
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Kang, Sanmao ; Liao, Pao-Chi ; Gage, Douglas A. ; Esselman, Walter J. / Identification of in vivo phosphorylation sites of CD45 protein-tyrosine phosphatase in 70Z/3.12 Cells. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 17. pp. 11588-11596.
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Identification of in vivo phosphorylation sites of CD45 protein-tyrosine phosphatase in 70Z/3.12 Cells. / Kang, Sanmao; Liao, Pao-Chi; Gage, Douglas A.; Esselman, Walter J.

In: Journal of Biological Chemistry, Vol. 272, No. 17, 25.04.1997, p. 11588-11596.

Research output: Contribution to journalArticle

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