Identification of minuscule inward currents as precursors to membrane electroporation-induced currents: Real-time prediction of pore appearance

Edmund Cheung So, Ke Li Tsai, Fang Tzu Wu, Ming Chun Hsu, King Chuen Wu, Sheng Nan Wu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background/Aims: The objective of this study is to examine the current signals in response to large hyperpolarizations with the aid of principal component analysis (PCA) to search for or even predict current fluctuations related to membrane electroporation-induced current (I MEP ). Methods: The characteristics of principal eigenvalues generated for I MEP and the current signals at 10 sec prior to the start of initial I MEP (I Pre ) were examined. As membrane hyperpolarizations were applied at 0.1 Hz, the appearance of I MEP coincided with the higher principal eigenvalues extracted in PCA. Results: Subsequent addition of LaCl 3 (100 μM) greatly reduced I MEP and associated principal eigenvalues. In real-time analysis for a single frame (i.e, 300 msec), in response to large hyperpolarization, multiple runs of heralded minuscule inward currents (I min ) occurring before large rise in current amplitudes were detected. With PCA, such heralded I min was noted to coincide with the extreme principal eigenvalues. The duration of I min together with large principal eigenvalues was influenced by different levels of membrane hyperpolarization. In GH3 cells, palmitoyl-L-carnitine (PALCAR), a long-chain acylcarnitine, effectively increased the I MEP amplitude with an EC 50 value of 2.4 μM. However, in PALCAR-treated cells, the I min together with higher principal eigenvalues disappeared, while in isoflurane-treated cells, I min occurring before large rise of current amplitude remained intact. Similarly, the PCA analysis from I Pre in RAW 264.6 macrophages showed the presence of herald I min accompanied by the extreme principal eigenvalues. Conclusion: It is clear from this study that these large principal eigenvalues are representative of MEP-associated formation of electropores. Therefore, different compositions around the surface membrane of cells may alter the appearance of I min followed by I MEP emergence.

Original languageEnglish
Pages (from-to)402-416
Number of pages15
JournalCellular Physiology and Biochemistry
Volume32
Issue number2
DOIs
Publication statusPublished - 2013 Sep

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Electroporation
Principal Component Analysis
Membranes
Carnitine
Isoflurane
Macrophages
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

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title = "Identification of minuscule inward currents as precursors to membrane electroporation-induced currents: Real-time prediction of pore appearance",
abstract = "Background/Aims: The objective of this study is to examine the current signals in response to large hyperpolarizations with the aid of principal component analysis (PCA) to search for or even predict current fluctuations related to membrane electroporation-induced current (I MEP ). Methods: The characteristics of principal eigenvalues generated for I MEP and the current signals at 10 sec prior to the start of initial I MEP (I Pre ) were examined. As membrane hyperpolarizations were applied at 0.1 Hz, the appearance of I MEP coincided with the higher principal eigenvalues extracted in PCA. Results: Subsequent addition of LaCl 3 (100 μM) greatly reduced I MEP and associated principal eigenvalues. In real-time analysis for a single frame (i.e, 300 msec), in response to large hyperpolarization, multiple runs of heralded minuscule inward currents (I min ) occurring before large rise in current amplitudes were detected. With PCA, such heralded I min was noted to coincide with the extreme principal eigenvalues. The duration of I min together with large principal eigenvalues was influenced by different levels of membrane hyperpolarization. In GH3 cells, palmitoyl-L-carnitine (PALCAR), a long-chain acylcarnitine, effectively increased the I MEP amplitude with an EC 50 value of 2.4 μM. However, in PALCAR-treated cells, the I min together with higher principal eigenvalues disappeared, while in isoflurane-treated cells, I min occurring before large rise of current amplitude remained intact. Similarly, the PCA analysis from I Pre in RAW 264.6 macrophages showed the presence of herald I min accompanied by the extreme principal eigenvalues. Conclusion: It is clear from this study that these large principal eigenvalues are representative of MEP-associated formation of electropores. Therefore, different compositions around the surface membrane of cells may alter the appearance of I min followed by I MEP emergence.",
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Identification of minuscule inward currents as precursors to membrane electroporation-induced currents : Real-time prediction of pore appearance. / So, Edmund Cheung; Tsai, Ke Li; Wu, Fang Tzu; Hsu, Ming Chun; Wu, King Chuen; Wu, Sheng Nan.

In: Cellular Physiology and Biochemistry, Vol. 32, No. 2, 09.2013, p. 402-416.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of minuscule inward currents as precursors to membrane electroporation-induced currents

T2 - Real-time prediction of pore appearance

AU - So, Edmund Cheung

AU - Tsai, Ke Li

AU - Wu, Fang Tzu

AU - Hsu, Ming Chun

AU - Wu, King Chuen

AU - Wu, Sheng Nan

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