TY - JOUR
T1 - Identification of novel tumor markers for oral squamous cell carcinoma using glycoproteomic analysis
AU - Chen, Yi Ting
AU - Chong, Yi Min
AU - Cheng, Chu Wen
AU - Ho, Chung Liang
AU - Tsai, Hung Wen
AU - Kasten, Frederick H.
AU - Chen, Yu Ling
AU - Chang, Chuan Fa
N1 - Funding Information:
We are grateful for the support from the Tissue Bank and Bioinformatics Core Lab, Research Center of Clinical Medicine, National Cheng Kung University Hospital.
Funding Information:
This work was supported by the National Science Council ( NSC 101-2321-B-006-008- ).
PY - 2013
Y1 - 2013
N2 - Background: Oral cancer, the largest subset of head and neck cancer, has become one of the most lethal malignancies during the last two decades. Although several diagnostic tools have been applied for the early detection of oral malignancies, it is still urgent to identify novel tumor markers. In this study, we explored the cell surface N-glycomes of primary cultured human oral keratinocytes (HOK), immortalized human gingival keratinocytes (SG cells), and oral squamous cell carcinoma (OC2). Methods: Enzymatically hydrolyzed cell surface N-glycans were analyzed by MALDI-TOF mass spectrometry. Results: High levels of fucosylated N-glycans, especially core-fucosylated N-glycans, were observed on the OC2 cell surface whereas the major N-glycans on SG and HOK cells were high mannose type. In addition, the mRNA expression level of fucosyltransferase 8 was elevated significantly in OC2 cells than in SG and HOK cells. Core-fucosylated glycoproteins of OC2 cells were then purified with lectin affinity chromatography and a key adhesion molecule in cancer cells, CD147, was identified. Finally, overexpression of cell surface CD147 was confirmed on OC2 cells and oral cancer tissues (tissue array). Conclusions: CD147 was discovered by glycoproteomic approaches and suggested to be a potential novel tumor marker for oral cancer diagnosis.
AB - Background: Oral cancer, the largest subset of head and neck cancer, has become one of the most lethal malignancies during the last two decades. Although several diagnostic tools have been applied for the early detection of oral malignancies, it is still urgent to identify novel tumor markers. In this study, we explored the cell surface N-glycomes of primary cultured human oral keratinocytes (HOK), immortalized human gingival keratinocytes (SG cells), and oral squamous cell carcinoma (OC2). Methods: Enzymatically hydrolyzed cell surface N-glycans were analyzed by MALDI-TOF mass spectrometry. Results: High levels of fucosylated N-glycans, especially core-fucosylated N-glycans, were observed on the OC2 cell surface whereas the major N-glycans on SG and HOK cells were high mannose type. In addition, the mRNA expression level of fucosyltransferase 8 was elevated significantly in OC2 cells than in SG and HOK cells. Core-fucosylated glycoproteins of OC2 cells were then purified with lectin affinity chromatography and a key adhesion molecule in cancer cells, CD147, was identified. Finally, overexpression of cell surface CD147 was confirmed on OC2 cells and oral cancer tissues (tissue array). Conclusions: CD147 was discovered by glycoproteomic approaches and suggested to be a potential novel tumor marker for oral cancer diagnosis.
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U2 - 10.1016/j.cca.2012.10.019
DO - 10.1016/j.cca.2012.10.019
M3 - Article
C2 - 23078850
AN - SCOPUS:84877619051
SN - 0009-8981
VL - 420
SP - 45
EP - 53
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
ER -
