Identification of Staphylococcus spp. and detection of mecA by an oligonucleotide array

Huan Wen Han, Hsien-Chang Chang, Tsung Chain Chang

Research output: Contribution to journalArticle

Abstract

Phenotypic identification of coagulase-negative staphylococci (CoNS) is difficult and many staphylococcal species carry mecA. This study developed an array that was able to detect mecA and identify 30 staphylococcal species by targeting the internal transcribed spacer regions. A total of 129 target reference strains (30 species) and 434 clinical isolates of staphylococci were analyzed. Gene sequencing of 16S rRNA, gap or tuf genes was the reference method for species identification. All reference strains (100%) were correctly identified, while the identification rates of clinical isolates of S. aureus and CoNS were 98.9% and 98%, respectively. The sensitivity and specificity for mecA detection were 99% and 100%, respectively, in S. aureus isolates, and both values were 100% in isolates of CoNS. The assay takes 6 h from a purified culture isolate, and so far it has not been performed directly on patient samples.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume86
Issue number1
DOIs
Publication statusPublished - 2016 Sep 1

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Oligonucleotide Array Sequence Analysis
Staphylococcus
Coagulase
Genes
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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abstract = "Phenotypic identification of coagulase-negative staphylococci (CoNS) is difficult and many staphylococcal species carry mecA. This study developed an array that was able to detect mecA and identify 30 staphylococcal species by targeting the internal transcribed spacer regions. A total of 129 target reference strains (30 species) and 434 clinical isolates of staphylococci were analyzed. Gene sequencing of 16S rRNA, gap or tuf genes was the reference method for species identification. All reference strains (100{\%}) were correctly identified, while the identification rates of clinical isolates of S. aureus and CoNS were 98.9{\%} and 98{\%}, respectively. The sensitivity and specificity for mecA detection were 99{\%} and 100{\%}, respectively, in S. aureus isolates, and both values were 100{\%} in isolates of CoNS. The assay takes 6 h from a purified culture isolate, and so far it has not been performed directly on patient samples.",
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Identification of Staphylococcus spp. and detection of mecA by an oligonucleotide array. / Han, Huan Wen; Chang, Hsien-Chang; Chang, Tsung Chain.

In: Diagnostic Microbiology and Infectious Disease, Vol. 86, No. 1, 01.09.2016, p. 23-29.

Research output: Contribution to journalArticle

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