Identification of sucrose-regulated genes in cultured rice cells using mRNA differential display

Ta Chien Tseng, Teh Huei Tsai, Ming Yong Lue, Hung tu Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to get more information about carbon metabolite regulation pathways, cloning and sequence analysis of sucrose-regulated genes from rice-suspension-cultured cells were performed. We used a new method, mRNA differential display, to screen differentially expressed genes under conditions of 3% and no sucrose in the cultured medium. Six candidate clones were identified and sequenced. Clones SI1 and SI2 were repressed by sucrose starvation, while clones SR1, SR2, SR3 and SR4 were induced by sucrose starvation. Nucleotide sequence analysis showed that clone SR2 has 94.8% homology to the salT gene, and clones SI1 and SR3 show 88.3 and 96.9% identity, respectively, to partial cDNA sequences in the GenBank database. The results suggest that mRNA differential display provides an easy and quick way to clone genes involved in the carbon metabolite regulation pathway.

Original languageEnglish
Pages (from-to)179-182
Number of pages4
JournalGene
Volume161
Issue number2
DOIs
Publication statusPublished - 1995 Aug 19

All Science Journal Classification (ASJC) codes

  • Genetics

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