TY - JOUR
T1 - Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue
AU - Lin, Ying Hung
AU - Lin, Yung Ming
AU - Teng, Yen Ni
AU - Hsieh, Tsui Yu Tracy
AU - Lin, Yi Shing
AU - Kuo, Pao Lin
N1 - Funding Information:
Supported by grants from the National Science Council of the Republic of China (NSC 91-2314-B-006-149, NSC 91-3112-B-006-008, NSC 92-3112-B-006-002, NSC 93-3112-B-006-004, and NSC 93-2314-B-006-078).
PY - 2006/12
Y1 - 2006/12
N2 - Objective: To identify novel genes that are down-regulated in the testicular tissue of infertile men. Design: Prospective study. Setting: University-based reproductive clinics and genetics laboratory. Patients: Nine patients with normal spermatogenesis, and 15 patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS). Intervention: Testicular samples of patients with the same histology were pooled for complementary DNA (cDNA) microarray analysis. Main Outcome Measure: Novel, down-regulated genes. Results: In total, 300 genes were significantly down-regulated in SCOS or MA samples, and 10 novel sterility-related genes were identified. Of the 10 novel genes, 6 genes (Hs.126780, Hs.553658, Hs.274135, Hs.268122, Hs.531701, and Hs.171130) encode proteins with predictable functional domains, and all these functional domains are believed to correlate with spermatogenesis and/or spermiogenesis. Conversely, the other 4 genes (Hs.351582, Hs.407480, Hs.552781, and Hs.355570) do not encompass known functional domains. Two genes (Hs.407480 and Hs.552781) lack mouse orthologues. Most novel genes showed a testis-specific expression pattern in both mice and humans. Reverse transcription-polymerase chain reaction (RT-PCR) showed three distinct types of developmental stage-dependent expressions of message ribonucleic acid (mRNA) for these novel genes in murine testes. Conclusion: These 10 novel genes provide targets to elucidate novel pathways involved in human spermatogenesis.
AB - Objective: To identify novel genes that are down-regulated in the testicular tissue of infertile men. Design: Prospective study. Setting: University-based reproductive clinics and genetics laboratory. Patients: Nine patients with normal spermatogenesis, and 15 patients with maturation arrest (MA) or Sertoli cell-only syndrome (SCOS). Intervention: Testicular samples of patients with the same histology were pooled for complementary DNA (cDNA) microarray analysis. Main Outcome Measure: Novel, down-regulated genes. Results: In total, 300 genes were significantly down-regulated in SCOS or MA samples, and 10 novel sterility-related genes were identified. Of the 10 novel genes, 6 genes (Hs.126780, Hs.553658, Hs.274135, Hs.268122, Hs.531701, and Hs.171130) encode proteins with predictable functional domains, and all these functional domains are believed to correlate with spermatogenesis and/or spermiogenesis. Conversely, the other 4 genes (Hs.351582, Hs.407480, Hs.552781, and Hs.355570) do not encompass known functional domains. Two genes (Hs.407480 and Hs.552781) lack mouse orthologues. Most novel genes showed a testis-specific expression pattern in both mice and humans. Reverse transcription-polymerase chain reaction (RT-PCR) showed three distinct types of developmental stage-dependent expressions of message ribonucleic acid (mRNA) for these novel genes in murine testes. Conclusion: These 10 novel genes provide targets to elucidate novel pathways involved in human spermatogenesis.
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U2 - 10.1016/j.fertnstert.2006.04.039
DO - 10.1016/j.fertnstert.2006.04.039
M3 - Article
C2 - 17074343
AN - SCOPUS:33751197489
SN - 0015-0282
VL - 86
SP - 1650
EP - 1658
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 6
ER -