TY - JOUR
T1 - Identification of transcriptome profiles and signaling pathways for the allelochemical juglone in rice roots
AU - Chi, Wen Chang
AU - Fu, Shih Feng
AU - Huang, Tsai Lien
AU - Chen, Yun An
AU - Chen, Chi Cien
AU - Huang, Hao Jen
N1 - Funding Information:
Acknowledgments This work was supported by research grants from National Science Council (NSC 98-2621-B-006-003-MY3) and a grant from the Ministry of Education, Taiwan (Landmark Project Grant for National Cheng Kung University’s Top-University Project, B024). Agilent DNA microarray assays were performed by the DNA Microarray Core Laboratory at the Institute of Plant and Microbial Biology, Academia Sinica. Expression profile and data mining involved the system provided by the Bioinformatics Core for Genomic Medicine and Biotechnology Development at National Cheng Kung University, supported by a National Science Council grant (NSC 97-3112-B-006 -011).
PY - 2011/12
Y1 - 2011/12
N2 - Juglone (5-hydroxy-1,4-naphthoquinone) is known allelochemical, but its molecular mode of action is not well understood. We found that juglone induced reactive oxygen species production and calcium accumulation. To gain more insight into these cellular responses, we performed large-scale analysis of the rice transcriptome during juglone stress. Exposure to juglone triggered changes in transcript levels of genes related to cell growth, cell wall formation, chemical detoxification, abiotic stress response and epigenesis. The most predominant transcription-factor families were AP2/ERF, HSF, NAC, C2H2, WRKY, MYB and GRAS. Gene expression profiling of juglone-treated rice roots revealed upregulated signaling and biosynthesis of abscisic acid and jasmonic acid and inactivation of gibberellic acid. In addition, juglone upregulated the expression of two calcium-dependent protein kinases (CDPKs), 6 mitogen-activated protein kinase (MAPK) genes and 1 MAPK gene and markedly increased the activities of a CDPK-like kinase and MAPKs. Further characterization of these juglone-responsive genes may be helpful for better understanding the mechanisms of allelochemical tolerance in plants.
AB - Juglone (5-hydroxy-1,4-naphthoquinone) is known allelochemical, but its molecular mode of action is not well understood. We found that juglone induced reactive oxygen species production and calcium accumulation. To gain more insight into these cellular responses, we performed large-scale analysis of the rice transcriptome during juglone stress. Exposure to juglone triggered changes in transcript levels of genes related to cell growth, cell wall formation, chemical detoxification, abiotic stress response and epigenesis. The most predominant transcription-factor families were AP2/ERF, HSF, NAC, C2H2, WRKY, MYB and GRAS. Gene expression profiling of juglone-treated rice roots revealed upregulated signaling and biosynthesis of abscisic acid and jasmonic acid and inactivation of gibberellic acid. In addition, juglone upregulated the expression of two calcium-dependent protein kinases (CDPKs), 6 mitogen-activated protein kinase (MAPK) genes and 1 MAPK gene and markedly increased the activities of a CDPK-like kinase and MAPKs. Further characterization of these juglone-responsive genes may be helpful for better understanding the mechanisms of allelochemical tolerance in plants.
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U2 - 10.1007/s11103-011-9841-6
DO - 10.1007/s11103-011-9841-6
M3 - Article
C2 - 22065257
AN - SCOPUS:84862952933
SN - 0167-4412
VL - 77
SP - 591
EP - 607
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 6
ER -