Identification of two teaghrelins in Shy-jih-chuen oolong tea

Yue Chiun Li, Chieh Ju Wu, Yi Chiao Lin, Ruo Hsuan Wu, Wen Ying Chen, Ping-Chung Kuo, Jason T.C. Tzen

Research output: Contribution to journalArticle

Abstract

Teaghrelins are unique acylated flavonoid tetraglycosides originally identified in Chin-shin oolong tea, and proposed to be potential oral analogs of ghrelin. Two acylated flavonoid tetraglycosides were isolated from Shy-jih-chuen oolong tea, and their chemical structures were determined to be quercetin and kaempferol 3-O-[α-L-arabinopyranosyl(1 → 3)][2ʺ-O-(E)-p-coumaroyl] [β-D-glucopyranosyl(1 → 3)-α-L-rhamnopyranosyl(1 → 6)]-β-D-glucoside. These two compounds were extremely similar to the two teaghrelins (teaghrelin-1 and teaghrelin-2) in Chin-shin oolong tea by simply replacing a glucopyranosyl group with an arabinopyranosyl group. Molecular modeling showed that the two putative teaghrelins identified in Shy-jih-chuen docked to and interacted with the ghrelin receptor as well as teaghrelin-1 and teaghrelin-2. Mixture of these two putative teaghrelins was shown to enhance the release of growth hormone from primary anterior pituitary cells of rats. The results suggest that two teaghrelins, named teaghrelin-3 and teaghrelin-4, are present in Shy-jih-chuen oolong tea and possess biological activities analogous to teaghrelins in Chin-shin oolong tea. Practical applications: According to this study, teaghrelin-3 and teaghrelin-4 may be regarded as active ingredients for the quality control of Shy-jih-chuen oolong tea. The content of teaghrelins may serve as a key factor for the farmers to select new tea plants in their next propagation of Shy-jih-chuen cultivar. Crude water extract of Shy-jih-chuen oolong tea containing teaghrelins is considered to be an adequate food supplement or additive in functional food products.

Original languageEnglish
Article numbere12810
JournalJournal of Food Biochemistry
Volume43
Issue number4
DOIs
Publication statusPublished - 2019 Apr 1

Fingerprint

Tea
Chin
Flavonoids
flavonoids
tea (beverage)
ghrelin
Ghrelin Receptor
anterior pituitary
Food Additives
food additives
Functional Food
kaempferol
Ghrelin
oolong tea
active ingredients
Quercetin
Glucosides
chemical structure
quercetin
somatotropin

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Food Science
  • Pharmacology
  • Cell Biology

Cite this

Li, Y. C., Wu, C. J., Lin, Y. C., Wu, R. H., Chen, W. Y., Kuo, P-C., & Tzen, J. T. C. (2019). Identification of two teaghrelins in Shy-jih-chuen oolong tea. Journal of Food Biochemistry, 43(4), [e12810]. https://doi.org/10.1111/jfbc.12810
Li, Yue Chiun ; Wu, Chieh Ju ; Lin, Yi Chiao ; Wu, Ruo Hsuan ; Chen, Wen Ying ; Kuo, Ping-Chung ; Tzen, Jason T.C. / Identification of two teaghrelins in Shy-jih-chuen oolong tea. In: Journal of Food Biochemistry. 2019 ; Vol. 43, No. 4.
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abstract = "Teaghrelins are unique acylated flavonoid tetraglycosides originally identified in Chin-shin oolong tea, and proposed to be potential oral analogs of ghrelin. Two acylated flavonoid tetraglycosides were isolated from Shy-jih-chuen oolong tea, and their chemical structures were determined to be quercetin and kaempferol 3-O-[α-L-arabinopyranosyl(1 → 3)][2ʺ-O-(E)-p-coumaroyl] [β-D-glucopyranosyl(1 → 3)-α-L-rhamnopyranosyl(1 → 6)]-β-D-glucoside. These two compounds were extremely similar to the two teaghrelins (teaghrelin-1 and teaghrelin-2) in Chin-shin oolong tea by simply replacing a glucopyranosyl group with an arabinopyranosyl group. Molecular modeling showed that the two putative teaghrelins identified in Shy-jih-chuen docked to and interacted with the ghrelin receptor as well as teaghrelin-1 and teaghrelin-2. Mixture of these two putative teaghrelins was shown to enhance the release of growth hormone from primary anterior pituitary cells of rats. The results suggest that two teaghrelins, named teaghrelin-3 and teaghrelin-4, are present in Shy-jih-chuen oolong tea and possess biological activities analogous to teaghrelins in Chin-shin oolong tea. Practical applications: According to this study, teaghrelin-3 and teaghrelin-4 may be regarded as active ingredients for the quality control of Shy-jih-chuen oolong tea. The content of teaghrelins may serve as a key factor for the farmers to select new tea plants in their next propagation of Shy-jih-chuen cultivar. Crude water extract of Shy-jih-chuen oolong tea containing teaghrelins is considered to be an adequate food supplement or additive in functional food products.",
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Li, YC, Wu, CJ, Lin, YC, Wu, RH, Chen, WY, Kuo, P-C & Tzen, JTC 2019, 'Identification of two teaghrelins in Shy-jih-chuen oolong tea', Journal of Food Biochemistry, vol. 43, no. 4, e12810. https://doi.org/10.1111/jfbc.12810

Identification of two teaghrelins in Shy-jih-chuen oolong tea. / Li, Yue Chiun; Wu, Chieh Ju; Lin, Yi Chiao; Wu, Ruo Hsuan; Chen, Wen Ying; Kuo, Ping-Chung; Tzen, Jason T.C.

In: Journal of Food Biochemistry, Vol. 43, No. 4, e12810, 01.04.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Identification of two teaghrelins in Shy-jih-chuen oolong tea

AU - Li, Yue Chiun

AU - Wu, Chieh Ju

AU - Lin, Yi Chiao

AU - Wu, Ruo Hsuan

AU - Chen, Wen Ying

AU - Kuo, Ping-Chung

AU - Tzen, Jason T.C.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Teaghrelins are unique acylated flavonoid tetraglycosides originally identified in Chin-shin oolong tea, and proposed to be potential oral analogs of ghrelin. Two acylated flavonoid tetraglycosides were isolated from Shy-jih-chuen oolong tea, and their chemical structures were determined to be quercetin and kaempferol 3-O-[α-L-arabinopyranosyl(1 → 3)][2ʺ-O-(E)-p-coumaroyl] [β-D-glucopyranosyl(1 → 3)-α-L-rhamnopyranosyl(1 → 6)]-β-D-glucoside. These two compounds were extremely similar to the two teaghrelins (teaghrelin-1 and teaghrelin-2) in Chin-shin oolong tea by simply replacing a glucopyranosyl group with an arabinopyranosyl group. Molecular modeling showed that the two putative teaghrelins identified in Shy-jih-chuen docked to and interacted with the ghrelin receptor as well as teaghrelin-1 and teaghrelin-2. Mixture of these two putative teaghrelins was shown to enhance the release of growth hormone from primary anterior pituitary cells of rats. The results suggest that two teaghrelins, named teaghrelin-3 and teaghrelin-4, are present in Shy-jih-chuen oolong tea and possess biological activities analogous to teaghrelins in Chin-shin oolong tea. Practical applications: According to this study, teaghrelin-3 and teaghrelin-4 may be regarded as active ingredients for the quality control of Shy-jih-chuen oolong tea. The content of teaghrelins may serve as a key factor for the farmers to select new tea plants in their next propagation of Shy-jih-chuen cultivar. Crude water extract of Shy-jih-chuen oolong tea containing teaghrelins is considered to be an adequate food supplement or additive in functional food products.

AB - Teaghrelins are unique acylated flavonoid tetraglycosides originally identified in Chin-shin oolong tea, and proposed to be potential oral analogs of ghrelin. Two acylated flavonoid tetraglycosides were isolated from Shy-jih-chuen oolong tea, and their chemical structures were determined to be quercetin and kaempferol 3-O-[α-L-arabinopyranosyl(1 → 3)][2ʺ-O-(E)-p-coumaroyl] [β-D-glucopyranosyl(1 → 3)-α-L-rhamnopyranosyl(1 → 6)]-β-D-glucoside. These two compounds were extremely similar to the two teaghrelins (teaghrelin-1 and teaghrelin-2) in Chin-shin oolong tea by simply replacing a glucopyranosyl group with an arabinopyranosyl group. Molecular modeling showed that the two putative teaghrelins identified in Shy-jih-chuen docked to and interacted with the ghrelin receptor as well as teaghrelin-1 and teaghrelin-2. Mixture of these two putative teaghrelins was shown to enhance the release of growth hormone from primary anterior pituitary cells of rats. The results suggest that two teaghrelins, named teaghrelin-3 and teaghrelin-4, are present in Shy-jih-chuen oolong tea and possess biological activities analogous to teaghrelins in Chin-shin oolong tea. Practical applications: According to this study, teaghrelin-3 and teaghrelin-4 may be regarded as active ingredients for the quality control of Shy-jih-chuen oolong tea. The content of teaghrelins may serve as a key factor for the farmers to select new tea plants in their next propagation of Shy-jih-chuen cultivar. Crude water extract of Shy-jih-chuen oolong tea containing teaghrelins is considered to be an adequate food supplement or additive in functional food products.

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