Identifying LRRC16B as an oncofetal gene with transforming enhancing capability using a combined bioinformatics and experimental approach

C. C. Hsu, C. W. Chiang, H. C. Cheng, W. T. Chang, C. Y. Chou, H. W. Tsai, C. T. Lee, Z. H. Wu, T. Y. Lee, A. Chao, N. H. Chow, C. L. Ho

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10 Citations (Scopus)

Abstract

Oncofetal genes are expressed in embryos or fetuses, are downregulated or undetectable in adult tissues, and then re-expressed in tumors. Known oncofetal genes, such as AFP, GCB, FGF18, IMP-1 and SOX1, often have important clinical applications or pivotal biological functions. To find new oncofetal-like genes, we used the public information of expressed sequence tags to systematically analyze gene expression patterns and identified a novel oncofetal-like gene, LRRC16B. It increased the proliferation, anchorage-independent growth and tumorigenesis of transformed cells in xenografts, possibly through its effects on cyclin B1 protein levels. These findings exemplify the feasibility of using bioinformatics to find new oncofetal-like genes and suggest that more genes with important functional roles will be uncovered in the candidate gene list.

Original languageEnglish
Pages (from-to)654-667
Number of pages14
JournalOncogene
Volume30
Issue number6
DOIs
Publication statusPublished - 2011 Feb 10

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All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

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