TY - CHAP
T1 - Imaging MS Analysis in Catharanthus roseus
AU - Yamamoto, Kotaro
AU - Takahashi, Katsutoshi
AU - O’Connor, Sarah E.
AU - Mimura, Tetsuro
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - To understand how the plant regulates metabolism, it is important to determine where metabolites localize in the tissues and cells. Single-cell level omics approaches in plants have shown remarkable development over the last several years, and this data has been instrumental in gene discovery efforts for enzymes and transporters involved in metabolism. For metabolomics, Imaging Mass Spectrometry (IMS) is a powerful tool to map the spatial distribution of molecules in the tissue. Here, we describe the methods which we used to reveal where secondary metabolites, primarily alkaloids, localize in Catharanthus roseus stem and leaf tissues.
AB - To understand how the plant regulates metabolism, it is important to determine where metabolites localize in the tissues and cells. Single-cell level omics approaches in plants have shown remarkable development over the last several years, and this data has been instrumental in gene discovery efforts for enzymes and transporters involved in metabolism. For metabolomics, Imaging Mass Spectrometry (IMS) is a powerful tool to map the spatial distribution of molecules in the tissue. Here, we describe the methods which we used to reveal where secondary metabolites, primarily alkaloids, localize in Catharanthus roseus stem and leaf tissues.
UR - http://www.scopus.com/inward/record.url?scp=85132621071&partnerID=8YFLogxK
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U2 - 10.1007/978-1-0716-2349-7_2
DO - 10.1007/978-1-0716-2349-7_2
M3 - Chapter
C2 - 35732934
AN - SCOPUS:85132621071
T3 - Methods in Molecular Biology
SP - 33
EP - 43
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -