Abstract
This study investigated the potential application of immobilized diesel-degrading consortia for diesel removal in aqueous environments. The microorganisms were entrapped using polyurethane-polyurea co-polymers, alginate, and activate carbon, and total petroleum hydrocarbon (TPH) degradability were evaluated under freshwater and seawater conditions with diesel oil. The results indicated that immobilized cells remained active after entrapment, but rapid diesel degradation occurred after sufficient suspended cells growth in aqueous medium, suggesting that entrapped cells continuously released freely suspended cells and suspended cells degraded diesel as well. Under phosphorous-sufficient conditions (P/TPH>35%), TPH degradation efficiency was achieved at 80% even at low nitrogen condition (N/TPH<10%). Under phosphorous-insufficient conditions (P/TPH<10%), the better degradation efficiency was obtained only at high nitrogen content (TPH:N > 100:7). The stoichiometric relationship for diesel degradation, nitrogen consumption, phosphorous consumption, biomass production was obtained (100:5:0.9:35). The results of repeated batch indicated that immobilized cells could be repeatedly used for diesel degradation in simulated groundwater and seawater environments for more than 360 days of operation. With a combination of copolymer, alginate, and activate carbon, the entrapped matrix presented advantages on high surface area, high porosity, and high mechanical strength for a long-term operation for diesel bioremediation in aqueous environments.
| Original language | English |
|---|---|
| Pages (from-to) | 62-72 |
| Number of pages | 11 |
| Journal | International Biodeterioration and Biodegradation |
| Volume | 124 |
| DOIs | |
| Publication status | Published - 2017 Oct 1 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Biomaterials
- Waste Management and Disposal
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Immobilization of diesel-degrading consortia for bioremediation of diesel-contaminated groundwater and seawater. / Chen, Chih Hung; Whang, Liang-Ming; Pan, Chi Liang; Yang, Chao Long; Liu, Pao Wen Grace.
In: International Biodeterioration and Biodegradation, Vol. 124, 01.10.2017, p. 62-72.Research output: Contribution to journal › Article
TY - JOUR
T1 - Immobilization of diesel-degrading consortia for bioremediation of diesel-contaminated groundwater and seawater
AU - Chen, Chih Hung
AU - Whang, Liang-Ming
AU - Pan, Chi Liang
AU - Yang, Chao Long
AU - Liu, Pao Wen Grace
PY - 2017/10/1
Y1 - 2017/10/1
N2 - This study investigated the potential application of immobilized diesel-degrading consortia for diesel removal in aqueous environments. The microorganisms were entrapped using polyurethane-polyurea co-polymers, alginate, and activate carbon, and total petroleum hydrocarbon (TPH) degradability were evaluated under freshwater and seawater conditions with diesel oil. The results indicated that immobilized cells remained active after entrapment, but rapid diesel degradation occurred after sufficient suspended cells growth in aqueous medium, suggesting that entrapped cells continuously released freely suspended cells and suspended cells degraded diesel as well. Under phosphorous-sufficient conditions (P/TPH>35%), TPH degradation efficiency was achieved at 80% even at low nitrogen condition (N/TPH<10%). Under phosphorous-insufficient conditions (P/TPH<10%), the better degradation efficiency was obtained only at high nitrogen content (TPH:N > 100:7). The stoichiometric relationship for diesel degradation, nitrogen consumption, phosphorous consumption, biomass production was obtained (100:5:0.9:35). The results of repeated batch indicated that immobilized cells could be repeatedly used for diesel degradation in simulated groundwater and seawater environments for more than 360 days of operation. With a combination of copolymer, alginate, and activate carbon, the entrapped matrix presented advantages on high surface area, high porosity, and high mechanical strength for a long-term operation for diesel bioremediation in aqueous environments.
AB - This study investigated the potential application of immobilized diesel-degrading consortia for diesel removal in aqueous environments. The microorganisms were entrapped using polyurethane-polyurea co-polymers, alginate, and activate carbon, and total petroleum hydrocarbon (TPH) degradability were evaluated under freshwater and seawater conditions with diesel oil. The results indicated that immobilized cells remained active after entrapment, but rapid diesel degradation occurred after sufficient suspended cells growth in aqueous medium, suggesting that entrapped cells continuously released freely suspended cells and suspended cells degraded diesel as well. Under phosphorous-sufficient conditions (P/TPH>35%), TPH degradation efficiency was achieved at 80% even at low nitrogen condition (N/TPH<10%). Under phosphorous-insufficient conditions (P/TPH<10%), the better degradation efficiency was obtained only at high nitrogen content (TPH:N > 100:7). The stoichiometric relationship for diesel degradation, nitrogen consumption, phosphorous consumption, biomass production was obtained (100:5:0.9:35). The results of repeated batch indicated that immobilized cells could be repeatedly used for diesel degradation in simulated groundwater and seawater environments for more than 360 days of operation. With a combination of copolymer, alginate, and activate carbon, the entrapped matrix presented advantages on high surface area, high porosity, and high mechanical strength for a long-term operation for diesel bioremediation in aqueous environments.
UR - http://www.scopus.com/inward/record.url?scp=85025447794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85025447794&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2017.07.001
DO - 10.1016/j.ibiod.2017.07.001
M3 - Article
AN - SCOPUS:85025447794
VL - 124
SP - 62
EP - 72
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
SN - 0964-8305
ER -