TY - JOUR
T1 - Immobilized cell fixed-bed bioreactor for wastewater decolorization
AU - Chen, Bor Yann
AU - Chen, Shan Yu
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial supports (NSC-89-2214-E-035-015, NSC 90-2214-E-197-003, and NSC 93-2214-E-197-002) of National Science Council of Republic of China. Some significant conclusions were initiated while one of the authors (BYC) was a research associate with the National Research Council (NRC), USA working in the National Risk Management Research Laboratory (NRMRL), US Environmental Protection Agency (EPA), Cincinnati, Ohio, USA.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/11
Y1 - 2005/11
N2 - Fixed-bed bioreactors using gel-entrapped cells of Pseudomonas luteola were utilized for azo-dye decolorization in continuous mode. The decolorization performance of the fixed-bed decolorizers was examined to investigate the effect of bed length, volumetric flow rate, dye loading rate, dye concentration in the feed, as well as the characteristics of matrix (i.e., calcium alginate (CA) and polyacrylamide (PAA)) for cell immobilization. With a constant feeding dye concentration of 50 mg/l, the beds with CA-immobilized cells had an optimal volumetric decolorization rate (νv,dye) of 30.6 mg/h/l and a specific decolorization rate (νs,dye) of 2.61 mg/g cell/h when HRT and dye loading rate was 1.12 h and 2.25 mg/h, respectively. In contrast, the beds with PAA cells reached an optimal νv,dye and νs,dye of 7.12 mg/h/l and 1.70 mg/g cell/h, respectively, at a much longer HRT (8.0 h) due to diffusion-control mechanism for decolorization. For CA-cell beds, the dependence of specific decolorization rate on feeding dye concentration (0-200 mg/l) could be described by typical Monod-type kinetics, while for those with PAA cells, the relationship eventually followed first-order kinetics. This apparent first-order kinetics of PAA-cell systems is very likely due to mass transfer resistance of entrapped cells. At approximately same biomass loading, the beds with CA cells seem to be more economically feasible than those with PAA cells due to significantly less mass transfer resistance and higher volumetric decolorization rates.
AB - Fixed-bed bioreactors using gel-entrapped cells of Pseudomonas luteola were utilized for azo-dye decolorization in continuous mode. The decolorization performance of the fixed-bed decolorizers was examined to investigate the effect of bed length, volumetric flow rate, dye loading rate, dye concentration in the feed, as well as the characteristics of matrix (i.e., calcium alginate (CA) and polyacrylamide (PAA)) for cell immobilization. With a constant feeding dye concentration of 50 mg/l, the beds with CA-immobilized cells had an optimal volumetric decolorization rate (νv,dye) of 30.6 mg/h/l and a specific decolorization rate (νs,dye) of 2.61 mg/g cell/h when HRT and dye loading rate was 1.12 h and 2.25 mg/h, respectively. In contrast, the beds with PAA cells reached an optimal νv,dye and νs,dye of 7.12 mg/h/l and 1.70 mg/g cell/h, respectively, at a much longer HRT (8.0 h) due to diffusion-control mechanism for decolorization. For CA-cell beds, the dependence of specific decolorization rate on feeding dye concentration (0-200 mg/l) could be described by typical Monod-type kinetics, while for those with PAA cells, the relationship eventually followed first-order kinetics. This apparent first-order kinetics of PAA-cell systems is very likely due to mass transfer resistance of entrapped cells. At approximately same biomass loading, the beds with CA cells seem to be more economically feasible than those with PAA cells due to significantly less mass transfer resistance and higher volumetric decolorization rates.
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U2 - 10.1016/j.procbio.2005.04.002
DO - 10.1016/j.procbio.2005.04.002
M3 - Article
AN - SCOPUS:24944521541
SN - 1359-5113
VL - 40
SP - 3434
EP - 3440
JO - Process Biochemistry
JF - Process Biochemistry
IS - 11
ER -