Immunogenicity of an adeno-vector vaccine expressing the F protein of a respiratory syncytial virus manufactured from serum-free suspension culture

Hsiao Yun Shao, Huai Sheng Hsu, Shu Ling Yu, Shang Rung Wu, Kai Chieh Hu, Ching Kun Chang, Chia Chyi Liu, Yen Hung Chow

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have developed an efficient cell culture process to scale up the production of a recombinant adenovirus that expresses the membrane-trunked fusion protein of respiratory syncytial virus (RSV; Ad-F0ΔTM). Adherent cells of human embryonic kidney (HEK) 293-derived cell, 293A, which supports the production of E1/E3-deleted Ad-F0ΔTM when cultured in the presence of fetal bovine serum (FBS), were adapted to suspension growth under serum-free medium. In doing so, we studied the immunogenicity of Ad-F0ΔTMsus, which propagated in a bioreactor that was cultured with serum-free suspension of 293A, in comparison with Ad-F0ΔTMadh, which was produced from parental 293A cells that were adherently cultured in medium containing FBS. The size and morphology of Ad-F0ΔTMsus and Ad-F0ΔTMadh virions were identical upon inspection with electron microscopy. The results showed that anti-F IgG and RSV-neutralizing titer were raised in the serum of both mice that were intranasally immunized twice with Ad-F0ΔTMsus or Ad-F0ΔTMadh at two-week injection intervals. Furthermore, the immune responses persisted for six months after vaccination. Activation of F protein-specific CD8+ T cell's epitope associated IFN-γ and IL-4 was induced in both Ad-F0ΔTMsus- and Ad-F0ΔTMadh, but not in Ad-LacZsus, -immunized mouse splenocytes. No vaccine-enhanced lung inflammation, airway mucus occlusion or eosinophils infiltration were observed in Ad-immunized mice followed by RSV challenge; however, these symptoms were observed following immunization with formalin-inactivated RSV vaccine. These results indicate that the safety and potency of Ad-F0ΔTM produced from either adherent cells or suspension and serum-free cells are the same.

Original languageEnglish
Pages (from-to)27-35
Number of pages9
JournalAntiviral Research
Volume130
DOIs
Publication statusPublished - 2016 Jun 1

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Respiratory Syncytial Viruses
Suspensions
Vaccines
Serum
Proteins
Respiratory Syncytial Virus Vaccines
Membrane Fusion Proteins
Inactivated Vaccines
T-Lymphocyte Epitopes
Serum-Free Culture Media
Bioreactors
Mucus
Eosinophils
Adenoviridae
Interleukin-4
Virion
Formaldehyde
Immunization
Pneumonia
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Virology

Cite this

Shao, Hsiao Yun ; Hsu, Huai Sheng ; Yu, Shu Ling ; Wu, Shang Rung ; Hu, Kai Chieh ; Chang, Ching Kun ; Liu, Chia Chyi ; Chow, Yen Hung. / Immunogenicity of an adeno-vector vaccine expressing the F protein of a respiratory syncytial virus manufactured from serum-free suspension culture. In: Antiviral Research. 2016 ; Vol. 130. pp. 27-35.
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Immunogenicity of an adeno-vector vaccine expressing the F protein of a respiratory syncytial virus manufactured from serum-free suspension culture. / Shao, Hsiao Yun; Hsu, Huai Sheng; Yu, Shu Ling; Wu, Shang Rung; Hu, Kai Chieh; Chang, Ching Kun; Liu, Chia Chyi; Chow, Yen Hung.

In: Antiviral Research, Vol. 130, 01.06.2016, p. 27-35.

Research output: Contribution to journalArticle

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AU - Shao, Hsiao Yun

AU - Hsu, Huai Sheng

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AU - Wu, Shang Rung

AU - Hu, Kai Chieh

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AU - Liu, Chia Chyi

AU - Chow, Yen Hung

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