TY - GEN
T1 - Immunogold nanoparticle combing surface-enhanced raman scattering method for protein a detection
AU - Lin, Chi Chang
AU - Chen, Jr Tzai
AU - Chen, Yan Fu
AU - Chang, Tsung Chain
AU - Chang, Hsien-Chang
PY - 2007/8/28
Y1 - 2007/8/28
N2 - An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and Surface-Enhanced Raman Spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (mercaptobenzoic, MBA) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-MBA, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and high-throughput screening of antibodies.
AB - An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and Surface-Enhanced Raman Spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (mercaptobenzoic, MBA) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-MBA, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and high-throughput screening of antibodies.
UR - http://www.scopus.com/inward/record.url?scp=34548120154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548120154&partnerID=8YFLogxK
U2 - 10.1109/NEMS.2007.352209
DO - 10.1109/NEMS.2007.352209
M3 - Conference contribution
AN - SCOPUS:34548120154
SN - 1424406102
SN - 9781424406104
T3 - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
SP - 1093
EP - 1097
BT - Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
T2 - 2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
Y2 - 16 January 2007 through 19 January 2007
ER -