Immunogold nanoparticle combing surface-enhanced raman scattering method for protein a detection

Chi Chang Lin, Jr Tzai Chen, Yan Fu Chen, Tsung Chain Chang, Hsien-Chang Chang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and Surface-Enhanced Raman Spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (mercaptobenzoic, MBA) that is coimmobilized with biospecific anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-MBA, which combing both electromagnetic field and chemical enhancement, was used to exploits the SERS-derived signal. A dynamic range of 2-3 orders of magnitude and 1-10 pg/mL of detection limitation of protein A were achieved. The results indicated this new technique could be used in clinical diagnostic applications with fast, high sensitivity and high-throughput screening of antibodies.

Original languageEnglish
Title of host publicationProceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
Pages1093-1097
Number of pages5
DOIs
Publication statusPublished - 2007 Aug 28
Event2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007 - Bangkok, Thailand
Duration: 2007 Jan 162007 Jan 19

Publication series

NameProceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007

Other

Other2007 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007
Country/TerritoryThailand
CityBangkok
Period07-01-1607-01-19

All Science Journal Classification (ASJC) codes

  • Computer Networks and Communications
  • Computer Science Applications
  • Electrical and Electronic Engineering

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