TY - JOUR
T1 - Immunolocalization of collagen types in the subsynovial connective tissue within the carpal tunnel in humans
AU - Oh, Jinrok
AU - Zhao, Chunfeng
AU - Amadio, Peter C.
AU - An, Kai Nan
AU - Zobitz, Mark E.
AU - Wold, Lester E.
N1 - Funding Information:
This study was funded by grants from NIH (NIAMS) and Mayo Foundation. We gratefully acknowledge the help of Anke M. Ettema, MD, who provided the light microscopic images.
PY - 2005/9
Y1 - 2005/9
N2 - The tenosynovium within the carpal tunnel consists of a single layer of synovial cells, which lines the bursae within the carpal tunnel, and the subsynovial connective tissue (SSCT), which contains the tendon vasculature and other structural elements. In this study, we used immunogold labeling to localize collagen types within the SSCT in three cadaver specimens and three patients with carpal tunnel syndrome. Positive labeling for collagen types I, III and VI was found with immunoelectron microscopy. Collagen types I and III were codistributed within the SSCT. Type VI was primarily located in microfibrillar structures between collagen bundles, between elastin and collagen bundles and between collagen bundles and cells. There was no difference in the distribution of collagen types when comparing cadaver specimens and carpal tunnel patients.
AB - The tenosynovium within the carpal tunnel consists of a single layer of synovial cells, which lines the bursae within the carpal tunnel, and the subsynovial connective tissue (SSCT), which contains the tendon vasculature and other structural elements. In this study, we used immunogold labeling to localize collagen types within the SSCT in three cadaver specimens and three patients with carpal tunnel syndrome. Positive labeling for collagen types I, III and VI was found with immunoelectron microscopy. Collagen types I and III were codistributed within the SSCT. Type VI was primarily located in microfibrillar structures between collagen bundles, between elastin and collagen bundles and between collagen bundles and cells. There was no difference in the distribution of collagen types when comparing cadaver specimens and carpal tunnel patients.
UR - http://www.scopus.com/inward/record.url?scp=24344431556&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24344431556&partnerID=8YFLogxK
U2 - 10.1016/j.orthres.2005.02.009
DO - 10.1016/j.orthres.2005.02.009
M3 - Article
C2 - 15921875
AN - SCOPUS:24344431556
SN - 0736-0266
VL - 23
SP - 1226
EP - 1231
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
IS - 5
ER -