TY - JOUR
T1 - Immunosuppressive activity of serum taken from a liver transplant recipient after withdrawal of immunosuppressants
AU - Hsu, Li Wen
AU - Goto, Shigeru
AU - Nakano, Toshiaki
AU - Lai, Chia Yun
AU - Lin, Yu Chun
AU - Kao, Ying Hsien
AU - Chen, Shu Hui
AU - Cheng, Yu Fan
AU - Jawan, Bruno
AU - Chiu, King Wah
AU - Chen, Chao Long
N1 - Funding Information:
This work was supported by program project grant NHRI-EX94-9228SP from the National Health Research Institute, and grants 94-2314-B-182A-072, 94-2314-B-182A-009, 94-2314-B-182A-077 from the National Science Council, Taiwan.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/2
Y1 - 2007/2
N2 - In orthotopic liver transplantation (OLT), tolerance is induced in a certain combination of donors and a recipient in rats and, in some clinical cases, rejection has not occurred in OLT patients after weaning off immunosuppression. However, this mechanism has not yet been elucidated. Among our cases of liver transplantation (LTx), one OLT patient (Patient A) has not required immunosuppressive drugs for the last 5 years, following post-transplant lymphoproliferative disease (PTLD). This patient's serum interleukin-2 levels were undetectable following withdrawal of immunosuppressants. The same serum taken after discontinuing the immunosuppressants inhibited concanavalin A blast cultured cells and up-regulated the IL-4/IFN-γ gene expression ratio. These results suggested that other proteins were induced following withdrawal of immunosuppressants. Proteomic assay demonstrated 12 differentiated spots exclusive to this patient where immunosuppressants have been discontinued. Haptoglobin, found to have immunosuppressive activity in vitro, may play an important role in the maintenance of drug-free tolerance as a natural immunological suppressor after cessation of immunosuppression. Proteomic analysis will allow us to develop a novel weaning protocol for patients on long-term immunosuppression to avoid major immunosuppressant-related complications.
AB - In orthotopic liver transplantation (OLT), tolerance is induced in a certain combination of donors and a recipient in rats and, in some clinical cases, rejection has not occurred in OLT patients after weaning off immunosuppression. However, this mechanism has not yet been elucidated. Among our cases of liver transplantation (LTx), one OLT patient (Patient A) has not required immunosuppressive drugs for the last 5 years, following post-transplant lymphoproliferative disease (PTLD). This patient's serum interleukin-2 levels were undetectable following withdrawal of immunosuppressants. The same serum taken after discontinuing the immunosuppressants inhibited concanavalin A blast cultured cells and up-regulated the IL-4/IFN-γ gene expression ratio. These results suggested that other proteins were induced following withdrawal of immunosuppressants. Proteomic assay demonstrated 12 differentiated spots exclusive to this patient where immunosuppressants have been discontinued. Haptoglobin, found to have immunosuppressive activity in vitro, may play an important role in the maintenance of drug-free tolerance as a natural immunological suppressor after cessation of immunosuppression. Proteomic analysis will allow us to develop a novel weaning protocol for patients on long-term immunosuppression to avoid major immunosuppressant-related complications.
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U2 - 10.1016/j.trim.2006.06.001
DO - 10.1016/j.trim.2006.06.001
M3 - Article
C2 - 17306745
AN - SCOPUS:33846904331
SN - 0966-3274
VL - 17
SP - 137
EP - 146
JO - Transplant Immunology
JF - Transplant Immunology
IS - 2
ER -