Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp. LU310

Xueping Ling, Jing Guo, Xiaoting Liu, Xia Zhang, Nan Wang, Yinghua Lu, I. Son Ng

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A new isolated Schizochytrium sp. LU310 from the mangrove forest of Wenzhou, China, was found as a high producing microalga of docosahexaenoic acid (DHA). In this study, the significant improvements for DHA fermentation by the batch mode in the baffled flasks (i.e. higher oxygen supply) were achieved. By applied the nitrogen-feeding strategy in 1000. mL baffled flasks, the biomass, DHA concentration and DHA productivity were increased by 110.4%, 117.9% and 110.4%, respectively. Moreover, DHA concentration of 21.06. g/L was obtained by feeding 15. g/L of glucose intermittently, which was an increase of 41.25% over that of the batch mode. Finally, an innovative strategy was carried out by intermittent feeding carbon and simultaneously feeding nitrogen. The maximum DHA concentration and DHA productivity in the fed-batch cultivation reached to 24.74. g/L and 241.5. mg/L/h, respectively.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalBioresource technology
Volume184
DOIs
Publication statusPublished - 2015 May 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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