TY - JOUR
T1 - Impact of carbon and nitrogen feeding strategy on high production of biomass and docosahexaenoic acid (DHA) by Schizochytrium sp. LU310
AU - Ling, Xueping
AU - Guo, Jing
AU - Liu, Xiaoting
AU - Zhang, Xia
AU - Wang, Nan
AU - Lu, Yinghua
AU - Ng, I. Son
N1 - Funding Information:
The authors are grateful to the National Natural Science Foundation of China (No. 41206115 ), the Natural Science Foundation of Fujian Province of China (No. 2013J01060 ) and the National High-Tech R&D Program of China (No. 2014AA021701 ).
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - A new isolated Schizochytrium sp. LU310 from the mangrove forest of Wenzhou, China, was found as a high producing microalga of docosahexaenoic acid (DHA). In this study, the significant improvements for DHA fermentation by the batch mode in the baffled flasks (i.e. higher oxygen supply) were achieved. By applied the nitrogen-feeding strategy in 1000. mL baffled flasks, the biomass, DHA concentration and DHA productivity were increased by 110.4%, 117.9% and 110.4%, respectively. Moreover, DHA concentration of 21.06. g/L was obtained by feeding 15. g/L of glucose intermittently, which was an increase of 41.25% over that of the batch mode. Finally, an innovative strategy was carried out by intermittent feeding carbon and simultaneously feeding nitrogen. The maximum DHA concentration and DHA productivity in the fed-batch cultivation reached to 24.74. g/L and 241.5. mg/L/h, respectively.
AB - A new isolated Schizochytrium sp. LU310 from the mangrove forest of Wenzhou, China, was found as a high producing microalga of docosahexaenoic acid (DHA). In this study, the significant improvements for DHA fermentation by the batch mode in the baffled flasks (i.e. higher oxygen supply) were achieved. By applied the nitrogen-feeding strategy in 1000. mL baffled flasks, the biomass, DHA concentration and DHA productivity were increased by 110.4%, 117.9% and 110.4%, respectively. Moreover, DHA concentration of 21.06. g/L was obtained by feeding 15. g/L of glucose intermittently, which was an increase of 41.25% over that of the batch mode. Finally, an innovative strategy was carried out by intermittent feeding carbon and simultaneously feeding nitrogen. The maximum DHA concentration and DHA productivity in the fed-batch cultivation reached to 24.74. g/L and 241.5. mg/L/h, respectively.
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U2 - 10.1016/j.biortech.2014.09.130
DO - 10.1016/j.biortech.2014.09.130
M3 - Article
C2 - 25451778
AN - SCOPUS:84928330701
SN - 0960-8524
VL - 184
SP - 139
EP - 147
JO - Agricultural Wastes
JF - Agricultural Wastes
ER -
