Impact of chlorine on the cell integrity and toxin release and degradation of colonial Microcystis

Jiajia Fan, La Rao, Yi Ting Chiu, Tsair-Fuh Lin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The occurrence of toxic cyanobacteria in drinking water sources is problematic for water authorities as they can impair drinking water quality. Chlorine as a commonly used oxidant in water treatment plants has shown the potential to lyse cyanobacterial cells, resulting in the release of secondary metabolites which are hard to be removed during conventional water treatment processes. The majority of cyanobacterial species such as Microcystis, often occur in colonial forms under natural conditions. However, previous studies have mainly focused on the influence of chlorination on individual cyanobacterial cells due to technique limitations. A syringe dispersion method combined with a fluorescence technique (SYTOX Green stain with flow cytometry), was successfully developed for the evaluation of cell integrity of colonial Microcystis. Chlorination of Microcystis-laden water was conducted at different chlorine dosages for different colonial sizes (<37, 37–270 and 270–550 μm). The results indicated that colonial Microcystis cells were more resistant to chlorine oxidation than individual cells, which may be attributed to protection from the cell-bound mucilage. There was a lag phase before cell rupture occurred and a Delayed Chick Watson Model describes the experimental data very well for the kinetics of cyanobacterial cell rupture. The growing colonial size caused increases in the lag phases but decreases in the cell lysis rates. Chlorination also induced the release of microcystins (MCs) from colonial Microcystis cells. In particular, increased levels of dissolved MCs were observed in Cheng Kung Lake (CKL) water. In summary, the reaction of chlorine with colonial cyanobacteria is more complicated than with individual cells. The efficiency of chlorine oxidation could be reduced by the cell-bound mucilage and natural water matrix. These observations may provide insights for water authorities to assess the risk to drinking water quality posed by chlorination under natural conditions.

Original languageEnglish
Pages (from-to)394-404
Number of pages11
JournalWater Research
Volume102
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Chlorine
toxin
Chlorination
chlorine
chlorination
Degradation
degradation
Potable water
mucilage
drinking water
lysis
Water
Water quality
rupture
cyanobacterium
water quality
oxidation
Syringes
Oxidation
water

All Science Journal Classification (ASJC) codes

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

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abstract = "The occurrence of toxic cyanobacteria in drinking water sources is problematic for water authorities as they can impair drinking water quality. Chlorine as a commonly used oxidant in water treatment plants has shown the potential to lyse cyanobacterial cells, resulting in the release of secondary metabolites which are hard to be removed during conventional water treatment processes. The majority of cyanobacterial species such as Microcystis, often occur in colonial forms under natural conditions. However, previous studies have mainly focused on the influence of chlorination on individual cyanobacterial cells due to technique limitations. A syringe dispersion method combined with a fluorescence technique (SYTOX Green stain with flow cytometry), was successfully developed for the evaluation of cell integrity of colonial Microcystis. Chlorination of Microcystis-laden water was conducted at different chlorine dosages for different colonial sizes (<37, 37–270 and 270–550 μm). The results indicated that colonial Microcystis cells were more resistant to chlorine oxidation than individual cells, which may be attributed to protection from the cell-bound mucilage. There was a lag phase before cell rupture occurred and a Delayed Chick Watson Model describes the experimental data very well for the kinetics of cyanobacterial cell rupture. The growing colonial size caused increases in the lag phases but decreases in the cell lysis rates. Chlorination also induced the release of microcystins (MCs) from colonial Microcystis cells. In particular, increased levels of dissolved MCs were observed in Cheng Kung Lake (CKL) water. In summary, the reaction of chlorine with colonial cyanobacteria is more complicated than with individual cells. The efficiency of chlorine oxidation could be reduced by the cell-bound mucilage and natural water matrix. These observations may provide insights for water authorities to assess the risk to drinking water quality posed by chlorination under natural conditions.",
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Impact of chlorine on the cell integrity and toxin release and degradation of colonial Microcystis. / Fan, Jiajia; Rao, La; Chiu, Yi Ting; Lin, Tsair-Fuh.

In: Water Research, Vol. 102, 01.10.2016, p. 394-404.

Research output: Contribution to journalArticle

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