Abstract
Differentiation of normal human bladder cell (SVHUC) between two different-grade bladder cancer cell lines (TSGH8301, grade II and TCCSUP, grade IV) was successful developed based on a dielectrophoresis (DEP) microchip with microcavity array and multilayer electrodes. Single cell could be firstly trapped in the microcavity by negative DEP force between top and middle electrodes without overlapping problem; then, the trapped cells were sensed its impedance by sweeping AC signal in between middle and bottom electrodes. As the experimental results, the impedance of higher-grade bladder cancer cells was smaller than the value of lower-grade bladder cancer cells, i.e., TCCSUP (grade IV) < TSGH8301 (grade II), and the impedance of normal bladder cell was much higher than the values of both cancer cell lines. Basically, the impedances of all kinds of cell lines were decreased with the delay time measured when cells were taken out of the incubator. The largest difference of impedance between normal cells and cancer cells occurred as the delay time reached 1 hour, furthermore, the ratio of impedance between cancer cells and normal cells measured at 1 KHz and 0.2 V were 54% and 22% for TSGH8301 and TCCSUP, respectively. Consequently, the possibility of impedance measurement for evaluation of cancer cells was first proposed and investigated; moreover, the microchip provides the potential of electrical sensing for in vitro diagnosis under single cell resolution.
| Original language | English |
|---|---|
| Title of host publication | IEEE Sensors 2009 Conference - SENSORS 2009 |
| Pages | 943-947 |
| Number of pages | 5 |
| DOIs | |
| Publication status | Published - 2009 Dec 1 |
| Event | IEEE Sensors 2009 Conference - SENSORS 2009 - Christchurch, New Zealand Duration: 2009 Oct 25 → 2009 Oct 28 |
Publication series
| Name | Proceedings of IEEE Sensors |
|---|
Other
| Other | IEEE Sensors 2009 Conference - SENSORS 2009 |
|---|---|
| Country | New Zealand |
| City | Christchurch |
| Period | 09-10-25 → 09-10-28 |
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All Science Journal Classification (ASJC) codes
- Electrical and Electronic Engineering
Cite this
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Impedance sensing of bladder cancer cells based on a single-cell-based DEP microchip. / Chuang, Cheng Hsin; Wei, Ching Hua; Hsu, You Ming; Huang, Huei Sheng; Hsiao, Fei Bin.
IEEE Sensors 2009 Conference - SENSORS 2009. 2009. p. 943-947 5398183 (Proceedings of IEEE Sensors).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Impedance sensing of bladder cancer cells based on a single-cell-based DEP microchip
AU - Chuang, Cheng Hsin
AU - Wei, Ching Hua
AU - Hsu, You Ming
AU - Huang, Huei Sheng
AU - Hsiao, Fei Bin
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Differentiation of normal human bladder cell (SVHUC) between two different-grade bladder cancer cell lines (TSGH8301, grade II and TCCSUP, grade IV) was successful developed based on a dielectrophoresis (DEP) microchip with microcavity array and multilayer electrodes. Single cell could be firstly trapped in the microcavity by negative DEP force between top and middle electrodes without overlapping problem; then, the trapped cells were sensed its impedance by sweeping AC signal in between middle and bottom electrodes. As the experimental results, the impedance of higher-grade bladder cancer cells was smaller than the value of lower-grade bladder cancer cells, i.e., TCCSUP (grade IV) < TSGH8301 (grade II), and the impedance of normal bladder cell was much higher than the values of both cancer cell lines. Basically, the impedances of all kinds of cell lines were decreased with the delay time measured when cells were taken out of the incubator. The largest difference of impedance between normal cells and cancer cells occurred as the delay time reached 1 hour, furthermore, the ratio of impedance between cancer cells and normal cells measured at 1 KHz and 0.2 V were 54% and 22% for TSGH8301 and TCCSUP, respectively. Consequently, the possibility of impedance measurement for evaluation of cancer cells was first proposed and investigated; moreover, the microchip provides the potential of electrical sensing for in vitro diagnosis under single cell resolution.
AB - Differentiation of normal human bladder cell (SVHUC) between two different-grade bladder cancer cell lines (TSGH8301, grade II and TCCSUP, grade IV) was successful developed based on a dielectrophoresis (DEP) microchip with microcavity array and multilayer electrodes. Single cell could be firstly trapped in the microcavity by negative DEP force between top and middle electrodes without overlapping problem; then, the trapped cells were sensed its impedance by sweeping AC signal in between middle and bottom electrodes. As the experimental results, the impedance of higher-grade bladder cancer cells was smaller than the value of lower-grade bladder cancer cells, i.e., TCCSUP (grade IV) < TSGH8301 (grade II), and the impedance of normal bladder cell was much higher than the values of both cancer cell lines. Basically, the impedances of all kinds of cell lines were decreased with the delay time measured when cells were taken out of the incubator. The largest difference of impedance between normal cells and cancer cells occurred as the delay time reached 1 hour, furthermore, the ratio of impedance between cancer cells and normal cells measured at 1 KHz and 0.2 V were 54% and 22% for TSGH8301 and TCCSUP, respectively. Consequently, the possibility of impedance measurement for evaluation of cancer cells was first proposed and investigated; moreover, the microchip provides the potential of electrical sensing for in vitro diagnosis under single cell resolution.
UR - http://www.scopus.com/inward/record.url?scp=77951124121&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951124121&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2009.5398183
DO - 10.1109/ICSENS.2009.5398183
M3 - Conference contribution
AN - SCOPUS:77951124121
SN - 9781424445486
T3 - Proceedings of IEEE Sensors
SP - 943
EP - 947
BT - IEEE Sensors 2009 Conference - SENSORS 2009
ER -