Impedimetric monitoring of cell attachment on interdigitated microelectrodes

Bin Wha Chang; Che Hsiung Chen; Shin Jyh Ding; David Chan Hen Chen; Hsien Chang Chang

doi:10.1016/S0925-4005(04)00414-9

Impedimetric monitoring of cell attachment on interdigitated microelectrodes

Bin Wha Chang, Che Hsiung Chen, Shin Jyh Ding, David Chan Hen Chen, Hsien Chang Chang

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

An on-line and real-time impedimetric system was developed to monitor and quantify cell attachment on interdigitated microelectrodes. Changes in admittance at frequency lower than 10 kHz were positively correlated with the extent of cell attachment (10² to 10⁷ cells/cm ²). As judged from the admittance change, the onset and the extent of cell attachment were facilitated when the electrodes were modified with RGD-C peptide, an artificial peptide with a high affinity toward cellulose surface. The system is useful in estimating seeding cell density and characterizing bio-compatible materials with suitable cell affinity.

Original language	English
Pages (from-to)	159-163
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	105
Issue number	2
DOIs	https://doi.org/10.1016/S0925-4005(04)00414-9
Publication status	Published - 2005 Mar 28

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/S0925-4005(04)00414-9

Cite this

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title = "Impedimetric monitoring of cell attachment on interdigitated microelectrodes",

abstract = "An on-line and real-time impedimetric system was developed to monitor and quantify cell attachment on interdigitated microelectrodes. Changes in admittance at frequency lower than 10 kHz were positively correlated with the extent of cell attachment (102 to 107 cells/cm 2). As judged from the admittance change, the onset and the extent of cell attachment were facilitated when the electrodes were modified with RGD-C peptide, an artificial peptide with a high affinity toward cellulose surface. The system is useful in estimating seeding cell density and characterizing bio-compatible materials with suitable cell affinity.",

author = "Chang, {Bin Wha} and Chen, {Che Hsiung} and Ding, {Shin Jyh} and Chen, {David Chan Hen} and Chang, {Hsien Chang}",

note = "Funding Information: The authors would like to thank the assistance of equipment with the center for bioscience and biotechnology-NCKU and the southern region MEMS research center of National Science Council (NSC) in Taiwan. Finance supported by the NSC fund (NSC90-2213-E-006-069 and NSC91-2213-E006-069) is gratefully acknowledged. ",

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AU - Chang, Bin Wha

AU - Chen, Che Hsiung

AU - Ding, Shin Jyh

AU - Chen, David Chan Hen

AU - Chang, Hsien Chang

N1 - Funding Information: The authors would like to thank the assistance of equipment with the center for bioscience and biotechnology-NCKU and the southern region MEMS research center of National Science Council (NSC) in Taiwan. Finance supported by the NSC fund (NSC90-2213-E-006-069 and NSC91-2213-E006-069) is gratefully acknowledged.

PY - 2005/3/28

Y1 - 2005/3/28

N2 - An on-line and real-time impedimetric system was developed to monitor and quantify cell attachment on interdigitated microelectrodes. Changes in admittance at frequency lower than 10 kHz were positively correlated with the extent of cell attachment (102 to 107 cells/cm 2). As judged from the admittance change, the onset and the extent of cell attachment were facilitated when the electrodes were modified with RGD-C peptide, an artificial peptide with a high affinity toward cellulose surface. The system is useful in estimating seeding cell density and characterizing bio-compatible materials with suitable cell affinity.

AB - An on-line and real-time impedimetric system was developed to monitor and quantify cell attachment on interdigitated microelectrodes. Changes in admittance at frequency lower than 10 kHz were positively correlated with the extent of cell attachment (102 to 107 cells/cm 2). As judged from the admittance change, the onset and the extent of cell attachment were facilitated when the electrodes were modified with RGD-C peptide, an artificial peptide with a high affinity toward cellulose surface. The system is useful in estimating seeding cell density and characterizing bio-compatible materials with suitable cell affinity.

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Impedimetric monitoring of cell attachment on interdigitated microelectrodes

Abstract

All Science Journal Classification (ASJC) codes

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