Impedimetric monitoring of IGF-1 protection of in vitro cortical neurons under ischemic conditions

Shun Ho Huang, Shu Ping Lin, Chih Kuo Liang, Jia-Jin Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Microelectrode arrays (MEAs) incorporated with the electric cell substrate impedance sensing (ECIS) technique provide a method for acquiring cellular electrophysiological information, which is useful for the time-course monitoring of cellular outgrowth and damage. This research utilizes the ECIS technique for monitoring the time-course impedimetric changes in normal and insulin-like growth factor 1 (IGF-1)-protected cortical neurons under the ischemic insult of oxygen glucose deprivation (OGD) created in a microperfusion environment. The neuronal apoptosis is reflected by the relatively low cell viability (28 ± 11.5 %) after 30-min OGD followed by 24 h of re-oxygenation. Also the hyperpolarization phase of mitochondrial membrane potential (MMP) occurs during 2 h of the re-oxygenation period. In contrast, cortical neurons treated with 50 and 100 ng/mL IGF-1 show higher survival rates of 45 ± 5.2 % and 49 ± 9.2 %, respectively, and no occurrence of the hyperpolarization of MMP during the re-oxygenation period. The ECIS results demonstrate that the measured impedance of cortical neurons decreased from 826 ± 86 kΩ to 224 ± 32 kΩ due to cell detachment under the insult of OGD. The measured impedance of IGF-1-protected cortical neurons slowly decreased to about 50 % of the original value (560 ± 45 kΩ for 50 ng/mL and 593 ± 44 kΩ for 100 ng/mL) compared to saline control of 232 ± 37 kΩ, which indicates improved cell adhesion under OGD conditions. The time-course impedimetric results show that the proposed ECIS-based MEAs platform incorporated with a microperfusion environment can be used for the real-time monitoring of cortical neurons under in vitro OGD and the IGF-1 protective effect against OGD-induced ischemic neuronal death.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalBiomedical Microdevices
Volume15
Issue number1
DOIs
Publication statusPublished - 2013 Feb 1

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Insulin
Somatomedins
Electric Impedance
Electric batteries
Neurons
Glucose
Oxygen
Oxygenation
Monitoring
Mitochondrial Membrane Potential
Microelectrodes
Substrates
Membranes
Cell adhesion
Cell death
Cell Adhesion
Intercellular Signaling Peptides and Proteins
In Vitro Techniques
Cell Survival
Cells

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Cite this

Huang, Shun Ho ; Lin, Shu Ping ; Liang, Chih Kuo ; Chen, Jia-Jin. / Impedimetric monitoring of IGF-1 protection of in vitro cortical neurons under ischemic conditions. In: Biomedical Microdevices. 2013 ; Vol. 15, No. 1. pp. 135-143.
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abstract = "Microelectrode arrays (MEAs) incorporated with the electric cell substrate impedance sensing (ECIS) technique provide a method for acquiring cellular electrophysiological information, which is useful for the time-course monitoring of cellular outgrowth and damage. This research utilizes the ECIS technique for monitoring the time-course impedimetric changes in normal and insulin-like growth factor 1 (IGF-1)-protected cortical neurons under the ischemic insult of oxygen glucose deprivation (OGD) created in a microperfusion environment. The neuronal apoptosis is reflected by the relatively low cell viability (28 ± 11.5 {\%}) after 30-min OGD followed by 24 h of re-oxygenation. Also the hyperpolarization phase of mitochondrial membrane potential (MMP) occurs during 2 h of the re-oxygenation period. In contrast, cortical neurons treated with 50 and 100 ng/mL IGF-1 show higher survival rates of 45 ± 5.2 {\%} and 49 ± 9.2 {\%}, respectively, and no occurrence of the hyperpolarization of MMP during the re-oxygenation period. The ECIS results demonstrate that the measured impedance of cortical neurons decreased from 826 ± 86 kΩ to 224 ± 32 kΩ due to cell detachment under the insult of OGD. The measured impedance of IGF-1-protected cortical neurons slowly decreased to about 50 {\%} of the original value (560 ± 45 kΩ for 50 ng/mL and 593 ± 44 kΩ for 100 ng/mL) compared to saline control of 232 ± 37 kΩ, which indicates improved cell adhesion under OGD conditions. The time-course impedimetric results show that the proposed ECIS-based MEAs platform incorporated with a microperfusion environment can be used for the real-time monitoring of cortical neurons under in vitro OGD and the IGF-1 protective effect against OGD-induced ischemic neuronal death.",
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Impedimetric monitoring of IGF-1 protection of in vitro cortical neurons under ischemic conditions. / Huang, Shun Ho; Lin, Shu Ping; Liang, Chih Kuo; Chen, Jia-Jin.

In: Biomedical Microdevices, Vol. 15, No. 1, 01.02.2013, p. 135-143.

Research output: Contribution to journalArticle

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