TY - JOUR
T1 - Importance of lighting color and period for CO2 fixation and C-phycocyanin production during Thermosynechococcus sp. CL-1 growth
AU - Narindri Rara Winayu, Birgitta
AU - Lin, Yu Ting
AU - Hsueh, Hsin Ta
AU - Chu, Hsin
N1 - Publisher Copyright:
© 2023 Elsevier Ltd
PY - 2024/1
Y1 - 2024/1
N2 - Lighting arrangement is a critical factor in cultivation of photosynthetic microorganisms such as a cyanobacterium, Thermosynechococcus sp. CL-1 (TCL-1). This study observed the impact of various light intensities, light colors, and lighting periods on TCL-1 CO2 fixation rate and C-phycocyanin production. TCL-1 growth and CO2 fixation rate were not affected by the light color during continuous illumination. The best CO2 fixation rate of 2.29 ± 0.54 and 2.26 ± 0.14 g-CO2/L/d were achieved under white and red lighting, respectively. However, application of 12/12 h light-dark illumination diminished TCL-1 performance especially under red light and it might be caused by the reduction of accumulated chlorophyll-a. Furthermore, extended studies were also conducted for the efficient C-phycocyanin extraction and purification strategies, along with accumulated cyanotoxins analysis. Purification using ion-exchange chromatography elevated C-phycocyanin purity index to 3.34. Besides, treatment using distilled water and freeze dryer could remove the accumulated cylindrospermopsin and saxitoxin from TCL-1.
AB - Lighting arrangement is a critical factor in cultivation of photosynthetic microorganisms such as a cyanobacterium, Thermosynechococcus sp. CL-1 (TCL-1). This study observed the impact of various light intensities, light colors, and lighting periods on TCL-1 CO2 fixation rate and C-phycocyanin production. TCL-1 growth and CO2 fixation rate were not affected by the light color during continuous illumination. The best CO2 fixation rate of 2.29 ± 0.54 and 2.26 ± 0.14 g-CO2/L/d were achieved under white and red lighting, respectively. However, application of 12/12 h light-dark illumination diminished TCL-1 performance especially under red light and it might be caused by the reduction of accumulated chlorophyll-a. Furthermore, extended studies were also conducted for the efficient C-phycocyanin extraction and purification strategies, along with accumulated cyanotoxins analysis. Purification using ion-exchange chromatography elevated C-phycocyanin purity index to 3.34. Besides, treatment using distilled water and freeze dryer could remove the accumulated cylindrospermopsin and saxitoxin from TCL-1.
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U2 - 10.1016/j.bcab.2023.103003
DO - 10.1016/j.bcab.2023.103003
M3 - Article
AN - SCOPUS:85181082588
SN - 1878-8181
VL - 55
JO - Biocatalysis and Agricultural Biotechnology
JF - Biocatalysis and Agricultural Biotechnology
M1 - 103003
ER -