Important Roles of Ring Finger Protein 112 in Embryonic Vascular Development and Brain Functions

Jen Hui Tsou; Ying Chen Yang; Ping Chieh Pao; Hui Ching Lin; Nai Kuei Huang; Shih Ting Lin; Kuei Sen Hsu; Che Ming Yeh; Kuen Haur Lee; Chu Jen Kuo; De Ming Yang; Jiann Her Lin; Wen Chang Chang; Yi Chao Lee

doi:10.1007/s12035-016-9812-7

Important Roles of Ring Finger Protein 112 in Embryonic Vascular Development and Brain Functions

Jen Hui Tsou, Ying Chen Yang, Ping Chieh Pao, Hui Ching Lin, Nai Kuei Huang, Shih Ting Lin, Kuei Sen Hsu, Che Ming Yeh, Kuen Haur Lee, Chu Jen Kuo, De Ming Yang, Jiann Her Lin, Wen Chang Chang, Yi Chao Lee

Institute of Basic Medical Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Rnf112 is a member of the RING finger protein family. The expression of Rnf112 is abundant in the brain and is regulated during brain development. Our previous study has revealed that Rnf112 can promote neuronal differentiation by inhibiting the progression of the cell cycle in cell models. In this study, we further revealed the important functions of Rnf112 in embryo development and in adult brain. Our data showed that most of the Rnf112^−/− embryos exhibited blood vascular defects and died in utero. Upon further investigation, we found that the survival rate of homozygous Rnf112 knockout mice in 129/sv and C57BL/6 mixed genetic background was increased. The survived newborns of Rnf112^−/− mice manifested growth retardation as indicated by smaller size and a reduced weight. Although the overall organization of the brain did not appear to be severely affected in Rnf112^−/− mice, using in vivo 3D MRI imaging, we found that when compared to wild-type littermates, brains of Rnf112^−/− mice were smaller. In addition, Rnf112^−/− mice displayed impairment of brain functions including motor balance, and spatial learning and memory. Our results provide important aspects for the study of Rnf112 gene functions.

Original language	English
Pages (from-to)	2286-2300
Number of pages	15
Journal	Molecular Neurobiology
Volume	54
Issue number	3
DOIs	https://doi.org/10.1007/s12035-016-9812-7
Publication status	Published - 2017 Apr 1

All Science Journal Classification (ASJC) codes

Neurology
Cellular and Molecular Neuroscience

Access to Document

10.1007/s12035-016-9812-7

Cite this

@article{23ce1fad932149f2a5b5c50a9e8bcd08,

title = "Important Roles of Ring Finger Protein 112 in Embryonic Vascular Development and Brain Functions",

abstract = "Rnf112 is a member of the RING finger protein family. The expression of Rnf112 is abundant in the brain and is regulated during brain development. Our previous study has revealed that Rnf112 can promote neuronal differentiation by inhibiting the progression of the cell cycle in cell models. In this study, we further revealed the important functions of Rnf112 in embryo development and in adult brain. Our data showed that most of the Rnf112−/− embryos exhibited blood vascular defects and died in utero. Upon further investigation, we found that the survival rate of homozygous Rnf112 knockout mice in 129/sv and C57BL/6 mixed genetic background was increased. The survived newborns of Rnf112−/− mice manifested growth retardation as indicated by smaller size and a reduced weight. Although the overall organization of the brain did not appear to be severely affected in Rnf112−/− mice, using in vivo 3D MRI imaging, we found that when compared to wild-type littermates, brains of Rnf112−/− mice were smaller. In addition, Rnf112−/− mice displayed impairment of brain functions including motor balance, and spatial learning and memory. Our results provide important aspects for the study of Rnf112 gene functions.",

author = "Tsou, {Jen Hui} and Yang, {Ying Chen} and Pao, {Ping Chieh} and Lin, {Hui Ching} and Huang, {Nai Kuei} and Lin, {Shih Ting} and Hsu, {Kuei Sen} and Yeh, {Che Ming} and Lee, {Kuen Haur} and Kuo, {Chu Jen} and Yang, {De Ming} and Lin, {Jiann Her} and Chang, {Wen Chang} and Lee, {Yi Chao}",

note = "Funding Information: This work was supported by the National Science Council, Republic of China (NSC 102-2320-B-038-024), Ministry of Science and Technology, Republic of China (MOST 103-2320-B-038-034, MOST 103-2320-B-197-002), and Taipei Medical University (TMU101-AE3-Y24). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank the technical services provided by the “Transgenic Mouse Model Core Facility of the National Core Facility Program for Biotechnology, National Science Council” and the “Gene Knockout Mouse Core Laboratory of National Taiwan University Center of Genomic Medicine.” We also thank the technical supports from the Taiwan Mouse Clinic (MOST 104-2325-B-001-011) which is funded by the National Research Program for Biopharmaceuticals (NRPB) at the Ministry of Science and Technology (MOST) of Taiwan. We thank Ms. Christine Chin-jung Hsieh for the English language editing of the manuscript. Publisher Copyright: {\textcopyright} 2016, Springer Science+Business Media New York.",

year = "2017",

month = apr,

day = "1",

doi = "10.1007/s12035-016-9812-7",

language = "English",

volume = "54",

pages = "2286--2300",

journal = "Molecular Neurobiology",

issn = "0893-7648",

publisher = "Humana Press",

number = "3",

}

TY - JOUR

T1 - Important Roles of Ring Finger Protein 112 in Embryonic Vascular Development and Brain Functions

AU - Tsou, Jen Hui

AU - Yang, Ying Chen

AU - Pao, Ping Chieh

AU - Lin, Hui Ching

AU - Huang, Nai Kuei

AU - Lin, Shih Ting

AU - Hsu, Kuei Sen

AU - Yeh, Che Ming

AU - Lee, Kuen Haur

AU - Kuo, Chu Jen

AU - Yang, De Ming

AU - Lin, Jiann Her

AU - Chang, Wen Chang

AU - Lee, Yi Chao

N1 - Funding Information: This work was supported by the National Science Council, Republic of China (NSC 102-2320-B-038-024), Ministry of Science and Technology, Republic of China (MOST 103-2320-B-038-034, MOST 103-2320-B-197-002), and Taipei Medical University (TMU101-AE3-Y24). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We thank the technical services provided by the “Transgenic Mouse Model Core Facility of the National Core Facility Program for Biotechnology, National Science Council” and the “Gene Knockout Mouse Core Laboratory of National Taiwan University Center of Genomic Medicine.” We also thank the technical supports from the Taiwan Mouse Clinic (MOST 104-2325-B-001-011) which is funded by the National Research Program for Biopharmaceuticals (NRPB) at the Ministry of Science and Technology (MOST) of Taiwan. We thank Ms. Christine Chin-jung Hsieh for the English language editing of the manuscript. Publisher Copyright: © 2016, Springer Science+Business Media New York.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Rnf112 is a member of the RING finger protein family. The expression of Rnf112 is abundant in the brain and is regulated during brain development. Our previous study has revealed that Rnf112 can promote neuronal differentiation by inhibiting the progression of the cell cycle in cell models. In this study, we further revealed the important functions of Rnf112 in embryo development and in adult brain. Our data showed that most of the Rnf112−/− embryos exhibited blood vascular defects and died in utero. Upon further investigation, we found that the survival rate of homozygous Rnf112 knockout mice in 129/sv and C57BL/6 mixed genetic background was increased. The survived newborns of Rnf112−/− mice manifested growth retardation as indicated by smaller size and a reduced weight. Although the overall organization of the brain did not appear to be severely affected in Rnf112−/− mice, using in vivo 3D MRI imaging, we found that when compared to wild-type littermates, brains of Rnf112−/− mice were smaller. In addition, Rnf112−/− mice displayed impairment of brain functions including motor balance, and spatial learning and memory. Our results provide important aspects for the study of Rnf112 gene functions.

AB - Rnf112 is a member of the RING finger protein family. The expression of Rnf112 is abundant in the brain and is regulated during brain development. Our previous study has revealed that Rnf112 can promote neuronal differentiation by inhibiting the progression of the cell cycle in cell models. In this study, we further revealed the important functions of Rnf112 in embryo development and in adult brain. Our data showed that most of the Rnf112−/− embryos exhibited blood vascular defects and died in utero. Upon further investigation, we found that the survival rate of homozygous Rnf112 knockout mice in 129/sv and C57BL/6 mixed genetic background was increased. The survived newborns of Rnf112−/− mice manifested growth retardation as indicated by smaller size and a reduced weight. Although the overall organization of the brain did not appear to be severely affected in Rnf112−/− mice, using in vivo 3D MRI imaging, we found that when compared to wild-type littermates, brains of Rnf112−/− mice were smaller. In addition, Rnf112−/− mice displayed impairment of brain functions including motor balance, and spatial learning and memory. Our results provide important aspects for the study of Rnf112 gene functions.

UR - http://www.scopus.com/inward/record.url?scp=84960088932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960088932&partnerID=8YFLogxK

U2 - 10.1007/s12035-016-9812-7

DO - 10.1007/s12035-016-9812-7

M3 - Article

C2 - 26951452

AN - SCOPUS:84960088932

SN - 0893-7648

VL - 54

SP - 2286

EP - 2300

JO - Molecular Neurobiology

JF - Molecular Neurobiology

IS - 3

ER -

Important Roles of Ring Finger Protein 112 in Embryonic Vascular Development and Brain Functions

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this