Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination

Chia Yuan Chang, Chun Yu Lin, Vladimir A. Hovhannisyan, Shean Jen Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 μm to 1.5 μm for a 50% improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanningbased TFMPM to 1.5 μm from optimal 3.0 μm of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.

Original languageEnglish
Title of host publicationAdvances in Microscopic Imaging II
EditorsEmmanuel Beaurepaire, Francesco Saverio Pavone
PublisherSPIE
ISBN (Electronic)9781510628458
DOIs
Publication statusPublished - 2019 Jan 1
EventAdvances in Microscopic Imaging II 2019 - Munich, Germany
Duration: 2019 Jun 262019 Jun 27

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11076
ISSN (Print)1605-7422

Conference

ConferenceAdvances in Microscopic Imaging II 2019
CountryGermany
CityMunich
Period19-06-2619-06-27

Fingerprint

Lighting
Microscopy
Microscopic examination
Microscopes
illumination
microscopes
microscopy
Imaging techniques
Digital devices
excitation
Scanning
Equipment and Supplies
scanning
Lenses
apertures
lenses
retarding
gratings
Scattering
Light

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Chang, C. Y., Lin, C. Y., Hovhannisyan, V. A., & Chen, S. J. (2019). Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination. In E. Beaurepaire, & F. S. Pavone (Eds.), Advances in Microscopic Imaging II [110760G] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11076). SPIE. https://doi.org/10.1117/12.2526908
Chang, Chia Yuan ; Lin, Chun Yu ; Hovhannisyan, Vladimir A. ; Chen, Shean Jen. / Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination. Advances in Microscopic Imaging II. editor / Emmanuel Beaurepaire ; Francesco Saverio Pavone. SPIE, 2019. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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abstract = "A developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 μm to 1.5 μm for a 50{\%} improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanningbased TFMPM to 1.5 μm from optimal 3.0 μm of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.",
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Chang, CY, Lin, CY, Hovhannisyan, VA & Chen, SJ 2019, Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination. in E Beaurepaire & FS Pavone (eds), Advances in Microscopic Imaging II., 110760G, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11076, SPIE, Advances in Microscopic Imaging II 2019, Munich, Germany, 19-06-26. https://doi.org/10.1117/12.2526908

Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination. / Chang, Chia Yuan; Lin, Chun Yu; Hovhannisyan, Vladimir A.; Chen, Shean Jen.

Advances in Microscopic Imaging II. ed. / Emmanuel Beaurepaire; Francesco Saverio Pavone. SPIE, 2019. 110760G (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11076).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chang CY, Lin CY, Hovhannisyan VA, Chen SJ. Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination. In Beaurepaire E, Pavone FS, editors, Advances in Microscopic Imaging II. SPIE. 2019. 110760G. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2526908