TY - GEN
T1 - Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination
AU - Chang, Chia Yuan
AU - Lin, Chun Yu
AU - Hovhannisyan, Vladimir A.
AU - Chen, Shean Jen
N1 - Publisher Copyright:
© SPIE-OSA 2019
PY - 2019
Y1 - 2019
N2 - A developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 µm to 1.5 µm for a 50% improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanning-based TFMPM to 1.5 µm from optimal 3.0 µm of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.
AB - A developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 µm to 1.5 µm for a 50% improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanning-based TFMPM to 1.5 µm from optimal 3.0 µm of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.
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U2 - 10.1117/12.2526908
DO - 10.1117/12.2526908
M3 - Conference contribution
AN - SCOPUS:85074250772
T3 - Optics InfoBase Conference Papers
BT - European Conference on Biomedical Optics, ECBO_2019
PB - OSA - The Optical Society
T2 - European Conference on Biomedical Optics, ECBO_2019
Y2 - 23 June 2019 through 25 June 2019
ER -