Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination

Chia Yuan Chang, Chun Yu Lin, Vladimir A. Hovhannisyan, Shean Jen Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A developed temporal focusing-based multiphoton microscope (TFMPM) has a digital micromirror device (DMD) which is adopted not only as a blazed grating for light spatial dispersion but also for patterned illumination simultaneously. Herein, the TFMPM has been extended to implement spatially modulated illumination at structured frequency and orientation to increase the beam coverage at the back-focal aperture of the objective lens. The axial excitation confinement (AEC) of TFMPM can be condensed from 3.0 µm to 1.5 µm for a 50% improvement. Furthermore, a multiline scanning mechanism based on the DMD can push its AEC nearly equivalent to line scanning-based TFMPM to 1.5 µm from optimal 3.0 µm of the conventional TFMPM. By using the TFMPM with structured illumination and multiline scanning, reconstructed biotissue images according to the condensed AEC structured illumination are shown obviously superior in contrast and better scattering suppression.

Original languageEnglish
Title of host publicationEuropean Conference on Biomedical Optics, ECBO_2019
PublisherOSA - The Optical Society
ISBN (Electronic)9781557528209
DOIs
Publication statusPublished - 2019 Jan 1
EventEuropean Conference on Biomedical Optics, ECBO_2019 - Munich, Netherlands
Duration: 2019 Jun 232019 Jun 25

Publication series

NameOptics InfoBase Conference Papers
VolumePart F142-ECBO 2019

Conference

ConferenceEuropean Conference on Biomedical Optics, ECBO_2019
CountryNetherlands
CityMunich
Period19-06-2319-06-25

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

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  • Cite this

    Chang, C. Y., Lin, C. Y., Hovhannisyan, V. A., & Chen, S. J. (2019). Improved and fast biotissue imaging by temporal focusing widefield multiphoton microscopy with spatially modulated illumination. In European Conference on Biomedical Optics, ECBO_2019 (Optics InfoBase Conference Papers; Vol. Part F142-ECBO 2019). OSA - The Optical Society. https://doi.org/10.1117/12.2526908