Improvement of outdoor culture efficiency of cyanobacteria by over-expression of stress tolerance genes and its implication as bio-refinery feedstock

Hsiang Yen Su; Hsiang Hui Chou; Te Jin Chow; Tse Min Lee; Jo Shu Chang; Wen Lii Huang; Hsien Jung Chen

doi:10.1016/j.biortech.2017.04.074

Improvement of outdoor culture efficiency of cyanobacteria by over-expression of stress tolerance genes and its implication as bio-refinery feedstock

Hsiang Yen Su, Hsiang Hui Chou, Te Jin Chow, Tse Min Lee, Jo Shu Chang, Wen Lii Huang, Hsien Jung Chen

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

This study was undertaken to increase the biomass and carbohydrate productivities of a freshwater cyanobacterium Synechococcus elongatus under hot outdoor conditions through genetic manipulation to facilitate the application of using the cyanobacterial biomass as bio-refinery feedstocks. The stress tolerance genes (hspA, osmotin) were expressed in S. elongatus to improve their growth under various environment stresses of outdoor cultivation. The results revealed that over-expression of hspA and osmotin significantly improved temperature (45 °C), high light intensity, and salt tolerances of S. elongatus cells, making it capable of efficiently growing in seawater under outdoor cultivation. The carbohydrate productivity of these stress tolerant strains was also 15–30-fold higher than that of the control strain, although the carbohydrate contents of the recombinant and control strains were similar. Our findings demonstrate that the genetic engineering for improved stresses tolerance in S. elongatus could facilitate the feasibility of using cyanobacteria as feedstock for bio-refinery industry.

Original language	English
Pages (from-to)	1294-1303
Number of pages	10
Journal	Bioresource technology
Volume	244
DOIs	https://doi.org/10.1016/j.biortech.2017.04.074
Publication status	Published - 2017

All Science Journal Classification (ASJC) codes

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2017.04.074

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title = "Improvement of outdoor culture efficiency of cyanobacteria by over-expression of stress tolerance genes and its implication as bio-refinery feedstock",

abstract = "This study was undertaken to increase the biomass and carbohydrate productivities of a freshwater cyanobacterium Synechococcus elongatus under hot outdoor conditions through genetic manipulation to facilitate the application of using the cyanobacterial biomass as bio-refinery feedstocks. The stress tolerance genes (hspA, osmotin) were expressed in S. elongatus to improve their growth under various environment stresses of outdoor cultivation. The results revealed that over-expression of hspA and osmotin significantly improved temperature (45 °C), high light intensity, and salt tolerances of S. elongatus cells, making it capable of efficiently growing in seawater under outdoor cultivation. The carbohydrate productivity of these stress tolerant strains was also 15–30-fold higher than that of the control strain, although the carbohydrate contents of the recombinant and control strains were similar. Our findings demonstrate that the genetic engineering for improved stresses tolerance in S. elongatus could facilitate the feasibility of using cyanobacteria as feedstock for bio-refinery industry.",

author = "Su, {Hsiang Yen} and Chou, {Hsiang Hui} and Chow, {Te Jin} and Lee, {Tse Min} and Chang, {Jo Shu} and Huang, {Wen Lii} and Chen, {Hsien Jung}",

note = "Funding Information: This work was supported by grants NSC103-2623-E-242-001-ET , MOST105-2221-E-242-002 , MOST103-3113-E-006-006 , MOST104-3113-E-006-003 , MOST105-3113-E-006-003 and MOST106-3113-E-006-011 from the Taiwan{\textquoteright}s Ministry of Science and Technology . Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

doi = "10.1016/j.biortech.2017.04.074",

language = "English",

volume = "244",

pages = "1294--1303",

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TY - JOUR

T1 - Improvement of outdoor culture efficiency of cyanobacteria by over-expression of stress tolerance genes and its implication as bio-refinery feedstock

AU - Su, Hsiang Yen

AU - Chou, Hsiang Hui

AU - Chow, Te Jin

AU - Lee, Tse Min

AU - Chang, Jo Shu

AU - Huang, Wen Lii

AU - Chen, Hsien Jung

N1 - Funding Information: This work was supported by grants NSC103-2623-E-242-001-ET , MOST105-2221-E-242-002 , MOST103-3113-E-006-006 , MOST104-3113-E-006-003 , MOST105-3113-E-006-003 and MOST106-3113-E-006-011 from the Taiwan’s Ministry of Science and Technology . Publisher Copyright: © 2017 Elsevier Ltd

PY - 2017

Y1 - 2017

N2 - This study was undertaken to increase the biomass and carbohydrate productivities of a freshwater cyanobacterium Synechococcus elongatus under hot outdoor conditions through genetic manipulation to facilitate the application of using the cyanobacterial biomass as bio-refinery feedstocks. The stress tolerance genes (hspA, osmotin) were expressed in S. elongatus to improve their growth under various environment stresses of outdoor cultivation. The results revealed that over-expression of hspA and osmotin significantly improved temperature (45 °C), high light intensity, and salt tolerances of S. elongatus cells, making it capable of efficiently growing in seawater under outdoor cultivation. The carbohydrate productivity of these stress tolerant strains was also 15–30-fold higher than that of the control strain, although the carbohydrate contents of the recombinant and control strains were similar. Our findings demonstrate that the genetic engineering for improved stresses tolerance in S. elongatus could facilitate the feasibility of using cyanobacteria as feedstock for bio-refinery industry.

AB - This study was undertaken to increase the biomass and carbohydrate productivities of a freshwater cyanobacterium Synechococcus elongatus under hot outdoor conditions through genetic manipulation to facilitate the application of using the cyanobacterial biomass as bio-refinery feedstocks. The stress tolerance genes (hspA, osmotin) were expressed in S. elongatus to improve their growth under various environment stresses of outdoor cultivation. The results revealed that over-expression of hspA and osmotin significantly improved temperature (45 °C), high light intensity, and salt tolerances of S. elongatus cells, making it capable of efficiently growing in seawater under outdoor cultivation. The carbohydrate productivity of these stress tolerant strains was also 15–30-fold higher than that of the control strain, although the carbohydrate contents of the recombinant and control strains were similar. Our findings demonstrate that the genetic engineering for improved stresses tolerance in S. elongatus could facilitate the feasibility of using cyanobacteria as feedstock for bio-refinery industry.

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Improvement of outdoor culture efficiency of cyanobacteria by over-expression of stress tolerance genes and its implication as bio-refinery feedstock

Abstract

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