Improvements in algal lipid production: a systems biology and gene editing approach

Avik Banerjee, Chiranjib Banerjee, Sangeeta Negi, Jo-Shu Chang, Pratyoosh Shukla

Research output: Contribution to journalReview article

17 Citations (Scopus)

Abstract

Background: In the wake of rising energy demands, microalgae have emerged as potential sources of sustainable and renewable carbon-neutral fuels, such as bio-hydrogen and bio-oil. Purpose: For rational metabolic engineering, the elucidation of metabolic pathways in fine detail and their manipulation according to requirements is the key to exploiting the use of microalgae. Emergence of site-specific nucleases have revolutionized applied research leading to biotechnological gains. Genome engineering as well as modulation of the endogenous genome with high precision using CRISPR systems is being gradually employed in microalgal research. Further, to optimize and produce better algal platforms, use of systems biology network analysis and integration of omics data is required. This review discusses two important approaches: systems biology and gene editing strategies used on microalgal systems with a focus on biofuel production and sustainable solutions. It also emphasizes that the integration of such systems would contribute and compliment applied research on microalgae. Conclusions: Recent advances in microalgae are discussed, including systems biology, gene editing approaches in lipid bio-synthesis, and antenna engineering. Lastly, it has been attempted here to showcase how CRISPR/Cas systems are a better editing tool than existing techniques that can be utilized for gene modulation and engineering during biofuel production.

Original languageEnglish
Pages (from-to)369-385
Number of pages17
JournalCritical Reviews in Biotechnology
Volume38
Issue number3
DOIs
Publication statusPublished - 2018 Apr 3

Fingerprint

Microalgae
Systems Biology
Lipids
Biofuels
CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats
Research
Genome
Systems Integration
Metabolic Engineering
Metabolic Networks and Pathways
Hydrogen
Oils
Carbon
Gene Editing
Genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Banerjee, Avik ; Banerjee, Chiranjib ; Negi, Sangeeta ; Chang, Jo-Shu ; Shukla, Pratyoosh. / Improvements in algal lipid production : a systems biology and gene editing approach. In: Critical Reviews in Biotechnology. 2018 ; Vol. 38, No. 3. pp. 369-385.
@article{6297f93bc96141c98d7866d8c6efcc42,
title = "Improvements in algal lipid production: a systems biology and gene editing approach",
abstract = "Background: In the wake of rising energy demands, microalgae have emerged as potential sources of sustainable and renewable carbon-neutral fuels, such as bio-hydrogen and bio-oil. Purpose: For rational metabolic engineering, the elucidation of metabolic pathways in fine detail and their manipulation according to requirements is the key to exploiting the use of microalgae. Emergence of site-specific nucleases have revolutionized applied research leading to biotechnological gains. Genome engineering as well as modulation of the endogenous genome with high precision using CRISPR systems is being gradually employed in microalgal research. Further, to optimize and produce better algal platforms, use of systems biology network analysis and integration of omics data is required. This review discusses two important approaches: systems biology and gene editing strategies used on microalgal systems with a focus on biofuel production and sustainable solutions. It also emphasizes that the integration of such systems would contribute and compliment applied research on microalgae. Conclusions: Recent advances in microalgae are discussed, including systems biology, gene editing approaches in lipid bio-synthesis, and antenna engineering. Lastly, it has been attempted here to showcase how CRISPR/Cas systems are a better editing tool than existing techniques that can be utilized for gene modulation and engineering during biofuel production.",
author = "Avik Banerjee and Chiranjib Banerjee and Sangeeta Negi and Jo-Shu Chang and Pratyoosh Shukla",
year = "2018",
month = "4",
day = "3",
doi = "10.1080/07388551.2017.1356803",
language = "English",
volume = "38",
pages = "369--385",
journal = "Critical Reviews in Biotechnology",
issn = "0738-8551",
publisher = "Informa Healthcare",
number = "3",

}

Improvements in algal lipid production : a systems biology and gene editing approach. / Banerjee, Avik; Banerjee, Chiranjib; Negi, Sangeeta; Chang, Jo-Shu; Shukla, Pratyoosh.

In: Critical Reviews in Biotechnology, Vol. 38, No. 3, 03.04.2018, p. 369-385.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Improvements in algal lipid production

T2 - a systems biology and gene editing approach

AU - Banerjee, Avik

AU - Banerjee, Chiranjib

AU - Negi, Sangeeta

AU - Chang, Jo-Shu

AU - Shukla, Pratyoosh

PY - 2018/4/3

Y1 - 2018/4/3

N2 - Background: In the wake of rising energy demands, microalgae have emerged as potential sources of sustainable and renewable carbon-neutral fuels, such as bio-hydrogen and bio-oil. Purpose: For rational metabolic engineering, the elucidation of metabolic pathways in fine detail and their manipulation according to requirements is the key to exploiting the use of microalgae. Emergence of site-specific nucleases have revolutionized applied research leading to biotechnological gains. Genome engineering as well as modulation of the endogenous genome with high precision using CRISPR systems is being gradually employed in microalgal research. Further, to optimize and produce better algal platforms, use of systems biology network analysis and integration of omics data is required. This review discusses two important approaches: systems biology and gene editing strategies used on microalgal systems with a focus on biofuel production and sustainable solutions. It also emphasizes that the integration of such systems would contribute and compliment applied research on microalgae. Conclusions: Recent advances in microalgae are discussed, including systems biology, gene editing approaches in lipid bio-synthesis, and antenna engineering. Lastly, it has been attempted here to showcase how CRISPR/Cas systems are a better editing tool than existing techniques that can be utilized for gene modulation and engineering during biofuel production.

AB - Background: In the wake of rising energy demands, microalgae have emerged as potential sources of sustainable and renewable carbon-neutral fuels, such as bio-hydrogen and bio-oil. Purpose: For rational metabolic engineering, the elucidation of metabolic pathways in fine detail and their manipulation according to requirements is the key to exploiting the use of microalgae. Emergence of site-specific nucleases have revolutionized applied research leading to biotechnological gains. Genome engineering as well as modulation of the endogenous genome with high precision using CRISPR systems is being gradually employed in microalgal research. Further, to optimize and produce better algal platforms, use of systems biology network analysis and integration of omics data is required. This review discusses two important approaches: systems biology and gene editing strategies used on microalgal systems with a focus on biofuel production and sustainable solutions. It also emphasizes that the integration of such systems would contribute and compliment applied research on microalgae. Conclusions: Recent advances in microalgae are discussed, including systems biology, gene editing approaches in lipid bio-synthesis, and antenna engineering. Lastly, it has been attempted here to showcase how CRISPR/Cas systems are a better editing tool than existing techniques that can be utilized for gene modulation and engineering during biofuel production.

UR - http://www.scopus.com/inward/record.url?scp=85027187513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027187513&partnerID=8YFLogxK

U2 - 10.1080/07388551.2017.1356803

DO - 10.1080/07388551.2017.1356803

M3 - Review article

C2 - 28793788

AN - SCOPUS:85027187513

VL - 38

SP - 369

EP - 385

JO - Critical Reviews in Biotechnology

JF - Critical Reviews in Biotechnology

SN - 0738-8551

IS - 3

ER -