TY - JOUR
T1 - Improving cell disruption efficiency to facilitate protein release from microalgae using chemical and mechanical integrated method
AU - Phong, Win Nee
AU - Show, Pau Loke
AU - Le, Cheng Foh
AU - Tao, Yang
AU - Chang, Jo Shu
AU - Ling, Tau Chuan
N1 - Funding Information:
This work was supported financially by SATU Joint Research Scheme ( RU018C-2016 , RU018L-2016 , RU022E-2014 ) from University of Malaya ( PG250-2015B ), Malaysia’s Fundamental Research Grant Scheme ( FRGS/1/2015/SG05/UNIM/03/1 ), Malaysia’s Ministry of Science, Technology, Innovation ( MOSTI-02-02-12-SF0256 ), Taiwan's Ministry of Science and Technology ( 104-3113-E-006-003 - and 103-2221-E-006-190-MY3 ) and Taiwan's Ministry of Education on Top University Grants .
Funding Information:
This work was supported financially by SATU Joint Research Scheme (RU018C-2016, RU018L-2016, RU022E-2014) from University of Malaya (PG250-2015B), Malaysia's Fundamental Research Grant Scheme (FRGS/1/2015/SG05/UNIM/03/1), Malaysia's Ministry of Science, Technology, Innovation (MOSTI-02-02-12-SF0256), Taiwan's Ministry of Science and Technology (104-3113-E-006-003- and 103-2221-E-006-190-MY3) and Taiwan's Ministry of Education on Top University Grants.
PY - 2018/7/15
Y1 - 2018/7/15
N2 - One of the critical challenges in releasing protein from microalgae is to effectively disrupt their rigid thick cell walls. This study could provide some guidance on the implementation of a simple, cost-effective and scalable cell disruption in a downstream processing of microalgal industry for the recovery of protein from microalgae. The effects of solvent types, alkalis, and ultrasonication in disrupting microalgal cell wall and protein solubility were studied. It was found that alkaline treatment played a key role in cell disruption and protein solubilisation. From the industrial perspective, water is an excellent choice of extractive solvent due to low-cost, safety, and scalability. Among all the tested methods, the combination of both alkaline and ultrasonication treatment demonstrated the greatest cell disruption efficiency and was thus suggested to be use at large scale.
AB - One of the critical challenges in releasing protein from microalgae is to effectively disrupt their rigid thick cell walls. This study could provide some guidance on the implementation of a simple, cost-effective and scalable cell disruption in a downstream processing of microalgal industry for the recovery of protein from microalgae. The effects of solvent types, alkalis, and ultrasonication in disrupting microalgal cell wall and protein solubility were studied. It was found that alkaline treatment played a key role in cell disruption and protein solubilisation. From the industrial perspective, water is an excellent choice of extractive solvent due to low-cost, safety, and scalability. Among all the tested methods, the combination of both alkaline and ultrasonication treatment demonstrated the greatest cell disruption efficiency and was thus suggested to be use at large scale.
UR - http://www.scopus.com/inward/record.url?scp=85045375881&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045375881&partnerID=8YFLogxK
U2 - 10.1016/j.bej.2018.04.002
DO - 10.1016/j.bej.2018.04.002
M3 - Article
AN - SCOPUS:85045375881
VL - 135
SP - 83
EP - 90
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
ER -