Improving vaccine potency through intercellular spreading and enhanced MHC class I presentation of antigen

C. F. Hung, W. F. Cheng, C. Y. Chai, K. F. Hsu, L. He, M. Ling, T. C. Wu

Research output: Contribution to journalArticlepeer-review

127 Citations (Scopus)

Abstract

The potency of naked DNA vaccines is limited by their inability to amplify and spread in vivo. VP22, a HSV-1 protein, has demonstrated the remarkable property of intercellular transport and may thus provide a unique approach for enhancing vaccine potency. Therefore, we created a novel fusion of VP22 with a model Ag, human papillomavirus type 16 E7, in a DNA vaccine that generated enhanced spreading and MHC class I presentation of Ag. These properties led to a dramatic increase in the number of E7-specific CD8+ T cell precursors in vaccinated mice (around 50-fold) and converted a less effective DNA vaccine into one with significant potency against E7-expressing tumors. In comparison, nonspreading VP221-267 mutants failed to enhance vaccine potency. Our data indicated that the potency of DNA vaccines may be dramatically improved through intercellular spreading and enhanced MHC class I presentation of Ag.

Original languageEnglish
Pages (from-to)5733-5740
Number of pages8
JournalJournal of Immunology
Volume166
Issue number9
DOIs
Publication statusPublished - 2001 May 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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