TY - JOUR
T1 - In situ encapsulation of antibody on TiO2 nanowire immunosensor via electro-polymerization of polypyrrole propylic acid
AU - Lin, Chi Chang
AU - Chu, Yung Ming
AU - Chang, Hsien Chang
N1 - Funding Information:
The authors thank Prof. Changming Li and Mr. Chunxian Guo of Division of BioEngineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore for providing Pa monomer. This work was co-supported by the Department of Biomedical Engineering, National Cheng Kung University, Taiwan and the Center for Advanced Bionanosystems, Nanyang Technological University, Singapore.
PY - 2013
Y1 - 2013
N2 - A conductance-based immunosensor was constructed based on an antibody/conducting polymer/TiO2 nanowire film. TiO2 nanowires (NWs) were made by the hydrothermal synthesis method and directly spin-coated on a gold microelectrode patterned surface. Conducting polymer polypyrrole propylic acid (PPa) and antibody composite films were electrochemically polymerized on patterned NWs using pyrrole propylic acid (Pa) and anti-rabbit IgG (1 Ab) mixture solutions. The surface properties in the designed immunosensor during the preparation processes of NWs, PPa (PPa/TiO 2 NWs), anti-rabbit IgG (PPa-1 Ab/TiO2 NWs immunosensor), and the measurement of rabbit IgG (2 Ab/PPa-1 Ab/TiO2 NWs immunosensor) were recorded and expressed by the changes in current-voltage (I-V). The devices designed in this study can detect 2 Ab within a linear range of 11.2 μg/mL to 112 μg/mL, and the detection sensitivity and R-square are -0.64A/(g/mL) and 0.896, respectively.
AB - A conductance-based immunosensor was constructed based on an antibody/conducting polymer/TiO2 nanowire film. TiO2 nanowires (NWs) were made by the hydrothermal synthesis method and directly spin-coated on a gold microelectrode patterned surface. Conducting polymer polypyrrole propylic acid (PPa) and antibody composite films were electrochemically polymerized on patterned NWs using pyrrole propylic acid (Pa) and anti-rabbit IgG (1 Ab) mixture solutions. The surface properties in the designed immunosensor during the preparation processes of NWs, PPa (PPa/TiO 2 NWs), anti-rabbit IgG (PPa-1 Ab/TiO2 NWs immunosensor), and the measurement of rabbit IgG (2 Ab/PPa-1 Ab/TiO2 NWs immunosensor) were recorded and expressed by the changes in current-voltage (I-V). The devices designed in this study can detect 2 Ab within a linear range of 11.2 μg/mL to 112 μg/mL, and the detection sensitivity and R-square are -0.64A/(g/mL) and 0.896, respectively.
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U2 - 10.1016/j.snb.2013.03.045
DO - 10.1016/j.snb.2013.03.045
M3 - Article
AN - SCOPUS:84885472030
VL - 187
SP - 533
EP - 539
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
ER -