In situ encapsulation of antibody on TiO2 nanowire immunosensor via electro-polymerization of polypyrrole propylic acid

Chi Chang Lin; Yung Ming Chu; Hsien Chang Chang

doi:10.1016/j.snb.2013.03.045

In situ encapsulation of antibody on TiO₂ nanowire immunosensor via electro-polymerization of polypyrrole propylic acid

Chi Chang Lin
, Yung Ming Chu
, Hsien Chang Chang

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

A conductance-based immunosensor was constructed based on an antibody/conducting polymer/TiO₂ nanowire film. TiO₂ nanowires (NWs) were made by the hydrothermal synthesis method and directly spin-coated on a gold microelectrode patterned surface. Conducting polymer polypyrrole propylic acid (PPa) and antibody composite films were electrochemically polymerized on patterned NWs using pyrrole propylic acid (Pa) and anti-rabbit IgG (1 Ab) mixture solutions. The surface properties in the designed immunosensor during the preparation processes of NWs, PPa (PPa/TiO ₂ NWs), anti-rabbit IgG (PPa-1 Ab/TiO₂ NWs immunosensor), and the measurement of rabbit IgG (2 Ab/PPa-1 Ab/TiO₂ NWs immunosensor) were recorded and expressed by the changes in current-voltage (I-V). The devices designed in this study can detect 2 Ab within a linear range of 11.2 μg/mL to 112 μg/mL, and the detection sensitivity and R-square are -0.64A/(g/mL) and 0.896, respectively.

Original language	English
Pages (from-to)	533-539
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	187
DOIs	https://doi.org/10.1016/j.snb.2013.03.045
Publication status	Published - 2013

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2013.03.045

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title = "In situ encapsulation of antibody on TiO2 nanowire immunosensor via electro-polymerization of polypyrrole propylic acid",

abstract = "A conductance-based immunosensor was constructed based on an antibody/conducting polymer/TiO2 nanowire film. TiO2 nanowires (NWs) were made by the hydrothermal synthesis method and directly spin-coated on a gold microelectrode patterned surface. Conducting polymer polypyrrole propylic acid (PPa) and antibody composite films were electrochemically polymerized on patterned NWs using pyrrole propylic acid (Pa) and anti-rabbit IgG (1 Ab) mixture solutions. The surface properties in the designed immunosensor during the preparation processes of NWs, PPa (PPa/TiO 2 NWs), anti-rabbit IgG (PPa-1 Ab/TiO2 NWs immunosensor), and the measurement of rabbit IgG (2 Ab/PPa-1 Ab/TiO2 NWs immunosensor) were recorded and expressed by the changes in current-voltage (I-V). The devices designed in this study can detect 2 Ab within a linear range of 11.2 μg/mL to 112 μg/mL, and the detection sensitivity and R-square are -0.64A/(g/mL) and 0.896, respectively.",

author = "Lin, \{Chi Chang\} and Chu, \{Yung Ming\} and Chang, \{Hsien Chang\}",

note = "Funding Information: The authors thank Prof. Changming Li and Mr. Chunxian Guo of Division of BioEngineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore for providing Pa monomer. This work was co-supported by the Department of Biomedical Engineering, National Cheng Kung University, Taiwan and the Center for Advanced Bionanosystems, Nanyang Technological University, Singapore. ",

year = "2013",

doi = "10.1016/j.snb.2013.03.045",

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AU - Lin, Chi Chang

AU - Chu, Yung Ming

AU - Chang, Hsien Chang

N1 - Funding Information: The authors thank Prof. Changming Li and Mr. Chunxian Guo of Division of BioEngineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore for providing Pa monomer. This work was co-supported by the Department of Biomedical Engineering, National Cheng Kung University, Taiwan and the Center for Advanced Bionanosystems, Nanyang Technological University, Singapore.

PY - 2013

Y1 - 2013

N2 - A conductance-based immunosensor was constructed based on an antibody/conducting polymer/TiO2 nanowire film. TiO2 nanowires (NWs) were made by the hydrothermal synthesis method and directly spin-coated on a gold microelectrode patterned surface. Conducting polymer polypyrrole propylic acid (PPa) and antibody composite films were electrochemically polymerized on patterned NWs using pyrrole propylic acid (Pa) and anti-rabbit IgG (1 Ab) mixture solutions. The surface properties in the designed immunosensor during the preparation processes of NWs, PPa (PPa/TiO 2 NWs), anti-rabbit IgG (PPa-1 Ab/TiO2 NWs immunosensor), and the measurement of rabbit IgG (2 Ab/PPa-1 Ab/TiO2 NWs immunosensor) were recorded and expressed by the changes in current-voltage (I-V). The devices designed in this study can detect 2 Ab within a linear range of 11.2 μg/mL to 112 μg/mL, and the detection sensitivity and R-square are -0.64A/(g/mL) and 0.896, respectively.

AB - A conductance-based immunosensor was constructed based on an antibody/conducting polymer/TiO2 nanowire film. TiO2 nanowires (NWs) were made by the hydrothermal synthesis method and directly spin-coated on a gold microelectrode patterned surface. Conducting polymer polypyrrole propylic acid (PPa) and antibody composite films were electrochemically polymerized on patterned NWs using pyrrole propylic acid (Pa) and anti-rabbit IgG (1 Ab) mixture solutions. The surface properties in the designed immunosensor during the preparation processes of NWs, PPa (PPa/TiO 2 NWs), anti-rabbit IgG (PPa-1 Ab/TiO2 NWs immunosensor), and the measurement of rabbit IgG (2 Ab/PPa-1 Ab/TiO2 NWs immunosensor) were recorded and expressed by the changes in current-voltage (I-V). The devices designed in this study can detect 2 Ab within a linear range of 11.2 μg/mL to 112 μg/mL, and the detection sensitivity and R-square are -0.64A/(g/mL) and 0.896, respectively.

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JO - Sensors and Actuators, B: Chemical

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ER -

In situ encapsulation of antibody on TiO₂ nanowire immunosensor via electro-polymerization of polypyrrole propylic acid

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