In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

Tien Chun Tsai, Chia Wei Liu, Yi Chen Wu, Neil Adrian P. Ondevilla, Masatoshi Osawa, Hsien-Chang Chang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

Original languageEnglish
Pages (from-to)300-305
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume175
DOIs
Publication statusPublished - 2019 Mar 1

Fingerprint

Ethyldimethylaminopropyl Carbodiimide
Infrared absorption
immobilization
Absorption spectroscopy
Immobilization
Gold
infrared absorption
Infrared spectroscopy
Spectrum Analysis
absorption spectroscopy
infrared spectroscopy
gold
phosphates
Phosphates
Ions
acids
Acids
ions
N-hydroxysuccinimide

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

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abstract = "In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).",
author = "Tsai, {Tien Chun} and Liu, {Chia Wei} and Wu, {Yi Chen} and Ondevilla, {Neil Adrian P.} and Masatoshi Osawa and Hsien-Chang Chang",
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journal = "Colloids and Surfaces B: Biointerfaces",
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In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS). / Tsai, Tien Chun; Liu, Chia Wei; Wu, Yi Chen; Ondevilla, Neil Adrian P.; Osawa, Masatoshi; Chang, Hsien-Chang.

In: Colloids and Surfaces B: Biointerfaces, Vol. 175, 01.03.2019, p. 300-305.

Research output: Contribution to journalArticle

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AU - Tsai, Tien Chun

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AU - Ondevilla, Neil Adrian P.

AU - Osawa, Masatoshi

AU - Chang, Hsien-Chang

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