TY - JOUR
T1 - In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)
AU - Tsai, Tien Chun
AU - Liu, Chia Wei
AU - Wu, Yi Chen
AU - Ondevilla, Neil Adrian P.
AU - Osawa, Masatoshi
AU - Chang, Hsien Chang
N1 - Funding Information:
This research was funded in part by the NCKU-Delta Collaboration Project with Delta Electronics. Additionally, this work was also supported by the Ministry of Science and Technology, R.O.C. (MOST 107-2218-E-006-011- and MOST 107-2811-E-006-514).
Funding Information:
This research was funded in part by the NCKU-Delta Collaboration Project with Delta Electronics . Additionally, this work was also supported by the Ministry of Science and Technology, R.O.C. ( MOST 107-2218-E-006-011- and MOST 107-2811-E-006-514 ).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).
AB - In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).
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U2 - 10.1016/j.colsurfb.2018.12.009
DO - 10.1016/j.colsurfb.2018.12.009
M3 - Article
C2 - 30553205
AN - SCOPUS:85058149402
SN - 0927-7765
VL - 175
SP - 300
EP - 305
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -