In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

Tien Chun Tsai; Chia Wei Liu; Yi Chen Wu; Neil Adrian P. Ondevilla; Masatoshi Osawa; Hsien Chang Chang

doi:10.1016/j.colsurfb.2018.12.009

In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

Tien Chun Tsai
, Chia Wei Liu
, Yi Chen Wu
, Neil Adrian P. Ondevilla
, Masatoshi Osawa
, Hsien Chang Chang

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

Original language	English
Pages (from-to)	300-305
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	175
DOIs	https://doi.org/10.1016/j.colsurfb.2018.12.009
Publication status	Published - 2019 Mar 1

All Science Journal Classification (ASJC) codes

Biotechnology
Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

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10.1016/j.colsurfb.2018.12.009

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title = "In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)",

abstract = "In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).",

author = "Tsai, \{Tien Chun\} and Liu, \{Chia Wei\} and Wu, \{Yi Chen\} and Ondevilla, \{Neil Adrian P.\} and Masatoshi Osawa and Chang, \{Hsien Chang\}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2019",

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doi = "10.1016/j.colsurfb.2018.12.009",

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T1 - In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

AU - Tsai, Tien Chun

AU - Liu, Chia Wei

AU - Wu, Yi Chen

AU - Ondevilla, Neil Adrian P.

AU - Osawa, Masatoshi

AU - Chang, Hsien Chang

PY - 2019/3/1

Y1 - 2019/3/1

N2 - In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

AB - In this study, the time-dependent reaction between 11-mercaptoundecanoic acid (11-MUA) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) is precisely characterized using surface enhanced infrared absorption spectroscopy (SEIRAS). According to the high correlation between the spectral results of SEIRAS and the electrochemical behavior, it strongly demonstrates that the EDC/NHS reaction would be obviously interfered by phosphate ions in the neutral pH condition (pH = 7.0).

UR - https://www.scopus.com/pages/publications/85058149402

UR - https://www.scopus.com/pages/publications/85058149402#tab=citedBy

U2 - 10.1016/j.colsurfb.2018.12.009

DO - 10.1016/j.colsurfb.2018.12.009

M3 - Article

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AN - SCOPUS:85058149402

SN - 0927-7765

VL - 175

SP - 300

EP - 305

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

ER -

In situ study of EDC/NHS immobilization on gold surface based on attenuated total reflection surface-enhanced infrared absorption spectroscopy (ATR-SEIRAS)

Abstract

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