In vitro and in vivo activity of meropenem and sulbactam against a multidrug-resistant Acinetobacter baumannii strain

Wen Chien Ko, Hsin Chun Lee, Shyh Ren Chiang, Jing Jou Yan, Jiunn Jong Wu, Chin Li Lu, Yin Ching Chuang

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72 Citations (Scopus)

Abstract

Objectives: The potential therapeutic role of meropenem combined with sulbactam against a clinical endemic isolate of multidrug-resistant Acinetobacter baumannii, Ab-153, was investigated. Methods: The antimicrobial susceptibility of Ab-153 to various drugs was studied by the agar dilution method and Etest strips. The antibacterial activity of meropenem and sulbactam were investigated by a time-kill study in vitro and further examined for therapeutic efficacy in vivo in a murine model. Results: In the time-kill study, at a concentration of 0.5 × MIC (4 mg/L) of meropenem, 1 × MIC (8 mg/L) of sulbactam and both in combination, only the combination demonstrated bactericidal effects and there was at least a 5 log10 reduction in bacterial colony counts after 48 h, compared with either drug alone. BALB/c mice infected with 2.1-2.6 × 107 cfu of Ab-153 were treated with 20 mg/kg meropenem every 8 h, 40mg/kg sulbactam every 8 h or both in combination. The survival rate of mice in the combination group was significantly higher than that in the meropenem-treated or sulbactam-treater group (87% versus 35%, P=0.0004; 87% versus 30%, P=0,0002). Conclusions: Meropenem in conjunction with sulbactam can exhibit more potent antimicrobial activity against Ab-153 than meropenem sulbactam alone.

Original languageEnglish
Pages (from-to)393-395
Number of pages3
JournalJournal of Antimicrobial Chemotherapy
Volume53
Issue number2
DOIs
Publication statusPublished - 2004 Feb

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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