In vitro and in vivo biological responses of plasma-sprayed hydroxyapatite coatings with posthydrothermal treatment

C. Y. Yang, T. M. Lee, C. W. Yang, L. R. Chen, M. C. Wu, T. S. Lui

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

This study was undertaken to evaluate the effect of post-hydrothermal treatment on the biological responses of the plasma-sprayed hydroxyapatite (HA)-coated Ti-6Al-4V implant system both in vitro and in vivo. After hydrothermal treatment, the HA coating (HAC) shows the high mechanical strength and indices-of-crystallinity, denser microstructure, lower concentrations of amorphous and impurity phases, when compared with the as-sprayed HAC. The in vitro cell-culture studies, using UMR106 osteoblast-like cell, demonstrated no signifiacnt cell growth on both surface of as-sprayed and hydrothermal-treated HACs during 10-day culture. The in vivo studies, using the transcortical implant model in the femora of goats, evaluated the histological responses of two coatings. After 6 week of implantation, using backscattered electron images, no substantial histological variations in the extents of new bone apposition and new bone healing between the two HACs were observed. However, the as-sprayed HAC, owing to the dissolution induced the granular particles dissociated from the HAC, showed the statically lower extent of new bone apposition than hydrothermal-treated HAC at 12 weeks. The results suggest that hydrothermal treatment could be used to improve the mechanical strength, crystallinity, and phase composition of HAC, which are important factors of long-term fixation and stability of implant. Besides, the treated HAC could also achieve the initial fixation of implant in clinical use.

Original languageEnglish
Pages (from-to)263-271
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume83
Issue number2
DOIs
Publication statusPublished - 2007 Nov 1

Fingerprint

Durapatite
Hydroxyapatite
Plasmas
Coatings
Bone
Sprayed coatings
Strength of materials
Osteoblasts
Cell growth
Cell culture
Phase composition
Dissolution
Impurities
Microstructure
Electrons

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

@article{99ca5efa9f074dd397bef5f6c8736fae,
title = "In vitro and in vivo biological responses of plasma-sprayed hydroxyapatite coatings with posthydrothermal treatment",
abstract = "This study was undertaken to evaluate the effect of post-hydrothermal treatment on the biological responses of the plasma-sprayed hydroxyapatite (HA)-coated Ti-6Al-4V implant system both in vitro and in vivo. After hydrothermal treatment, the HA coating (HAC) shows the high mechanical strength and indices-of-crystallinity, denser microstructure, lower concentrations of amorphous and impurity phases, when compared with the as-sprayed HAC. The in vitro cell-culture studies, using UMR106 osteoblast-like cell, demonstrated no signifiacnt cell growth on both surface of as-sprayed and hydrothermal-treated HACs during 10-day culture. The in vivo studies, using the transcortical implant model in the femora of goats, evaluated the histological responses of two coatings. After 6 week of implantation, using backscattered electron images, no substantial histological variations in the extents of new bone apposition and new bone healing between the two HACs were observed. However, the as-sprayed HAC, owing to the dissolution induced the granular particles dissociated from the HAC, showed the statically lower extent of new bone apposition than hydrothermal-treated HAC at 12 weeks. The results suggest that hydrothermal treatment could be used to improve the mechanical strength, crystallinity, and phase composition of HAC, which are important factors of long-term fixation and stability of implant. Besides, the treated HAC could also achieve the initial fixation of implant in clinical use.",
author = "Yang, {C. Y.} and Lee, {T. M.} and Yang, {C. W.} and Chen, {L. R.} and Wu, {M. C.} and Lui, {T. S.}",
year = "2007",
month = "11",
day = "1",
doi = "10.1002/jbm.a.31246",
language = "English",
volume = "83",
pages = "263--271",
journal = "Journal of Biomedical Materials Research",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

In vitro and in vivo biological responses of plasma-sprayed hydroxyapatite coatings with posthydrothermal treatment. / Yang, C. Y.; Lee, T. M.; Yang, C. W.; Chen, L. R.; Wu, M. C.; Lui, T. S.

In: Journal of Biomedical Materials Research - Part A, Vol. 83, No. 2, 01.11.2007, p. 263-271.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro and in vivo biological responses of plasma-sprayed hydroxyapatite coatings with posthydrothermal treatment

AU - Yang, C. Y.

AU - Lee, T. M.

AU - Yang, C. W.

AU - Chen, L. R.

AU - Wu, M. C.

AU - Lui, T. S.

PY - 2007/11/1

Y1 - 2007/11/1

N2 - This study was undertaken to evaluate the effect of post-hydrothermal treatment on the biological responses of the plasma-sprayed hydroxyapatite (HA)-coated Ti-6Al-4V implant system both in vitro and in vivo. After hydrothermal treatment, the HA coating (HAC) shows the high mechanical strength and indices-of-crystallinity, denser microstructure, lower concentrations of amorphous and impurity phases, when compared with the as-sprayed HAC. The in vitro cell-culture studies, using UMR106 osteoblast-like cell, demonstrated no signifiacnt cell growth on both surface of as-sprayed and hydrothermal-treated HACs during 10-day culture. The in vivo studies, using the transcortical implant model in the femora of goats, evaluated the histological responses of two coatings. After 6 week of implantation, using backscattered electron images, no substantial histological variations in the extents of new bone apposition and new bone healing between the two HACs were observed. However, the as-sprayed HAC, owing to the dissolution induced the granular particles dissociated from the HAC, showed the statically lower extent of new bone apposition than hydrothermal-treated HAC at 12 weeks. The results suggest that hydrothermal treatment could be used to improve the mechanical strength, crystallinity, and phase composition of HAC, which are important factors of long-term fixation and stability of implant. Besides, the treated HAC could also achieve the initial fixation of implant in clinical use.

AB - This study was undertaken to evaluate the effect of post-hydrothermal treatment on the biological responses of the plasma-sprayed hydroxyapatite (HA)-coated Ti-6Al-4V implant system both in vitro and in vivo. After hydrothermal treatment, the HA coating (HAC) shows the high mechanical strength and indices-of-crystallinity, denser microstructure, lower concentrations of amorphous and impurity phases, when compared with the as-sprayed HAC. The in vitro cell-culture studies, using UMR106 osteoblast-like cell, demonstrated no signifiacnt cell growth on both surface of as-sprayed and hydrothermal-treated HACs during 10-day culture. The in vivo studies, using the transcortical implant model in the femora of goats, evaluated the histological responses of two coatings. After 6 week of implantation, using backscattered electron images, no substantial histological variations in the extents of new bone apposition and new bone healing between the two HACs were observed. However, the as-sprayed HAC, owing to the dissolution induced the granular particles dissociated from the HAC, showed the statically lower extent of new bone apposition than hydrothermal-treated HAC at 12 weeks. The results suggest that hydrothermal treatment could be used to improve the mechanical strength, crystallinity, and phase composition of HAC, which are important factors of long-term fixation and stability of implant. Besides, the treated HAC could also achieve the initial fixation of implant in clinical use.

UR - http://www.scopus.com/inward/record.url?scp=35048872849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35048872849&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.31246

DO - 10.1002/jbm.a.31246

M3 - Article

C2 - 17415765

AN - SCOPUS:35048872849

VL - 83

SP - 263

EP - 271

JO - Journal of Biomedical Materials Research

JF - Journal of Biomedical Materials Research

SN - 1549-3296

IS - 2

ER -