In vitro and in vivo characterization of SU-8 flexible neuroprobe: From mechanical properties to electrophysiological recording

Shun Ho Huang, Shu Ping Lin, Jia-Jin Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Flexible neuroprobe with better tissue compliance exhibits unique mechanical characteristics in maintaining stability of neural implant in vivo. In this study, a flexible neuroprobe using SU-8 was designed and fabricated for in vitro and in vivo electrical sensing to show the improved tissue compatibility compared to that of the traditional rigid neuroprobe. The validation of neuroprobe was achieved by in vitro mechanical and cytotoxicity tests as well as in vivo neural recording and immunohistological staining. The fabrication process consisted of the creation of a backbone structure using photolithography, photopatterning of evaporated metal, and insulating of the electrode trace. The results of mechanical test of our fabricated SU-8 neuroprobe showed four times of physical stress (18.77 mN) than the insertion force (4.69 mN) to sustain resistance from brain tissue during implantation. The in vitro cytotoxicity assay showed well neuronal survival and proved the sufficient surface biocompatibility of the SU-8 neuroprobe. Further in vivo immunohistological staining showed no obvious glia aggregation around the implantation site indicating suitable biocompatibility compared with that of a rigid neuroprobe. Our in vitro and in vivo studies showed SU-8 neuroprobe possessed enough stress to complete the implantation in brain tissue and remained flexibility to comply micromovement of soft tissue with minor immune responses to achieve in vivo electrophysiological recordings at a signal-noise-ratio of greater than 7.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalSensors and Actuators, A: Physical
Volume216
DOIs
Publication statusPublished - 2014 Sep 1

Fingerprint

recording
mechanical properties
Tissue
Mechanical properties
implantation
biocompatibility
staining
Cytotoxicity
Biocompatibility
brain
Brain
Photolithography
photolithography
compatibility
insertion
Assays
flexibility
Agglomeration
Metals
Fabrication

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering

Cite this

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abstract = "Flexible neuroprobe with better tissue compliance exhibits unique mechanical characteristics in maintaining stability of neural implant in vivo. In this study, a flexible neuroprobe using SU-8 was designed and fabricated for in vitro and in vivo electrical sensing to show the improved tissue compatibility compared to that of the traditional rigid neuroprobe. The validation of neuroprobe was achieved by in vitro mechanical and cytotoxicity tests as well as in vivo neural recording and immunohistological staining. The fabrication process consisted of the creation of a backbone structure using photolithography, photopatterning of evaporated metal, and insulating of the electrode trace. The results of mechanical test of our fabricated SU-8 neuroprobe showed four times of physical stress (18.77 mN) than the insertion force (4.69 mN) to sustain resistance from brain tissue during implantation. The in vitro cytotoxicity assay showed well neuronal survival and proved the sufficient surface biocompatibility of the SU-8 neuroprobe. Further in vivo immunohistological staining showed no obvious glia aggregation around the implantation site indicating suitable biocompatibility compared with that of a rigid neuroprobe. Our in vitro and in vivo studies showed SU-8 neuroprobe possessed enough stress to complete the implantation in brain tissue and remained flexibility to comply micromovement of soft tissue with minor immune responses to achieve in vivo electrophysiological recordings at a signal-noise-ratio of greater than 7.",
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In vitro and in vivo characterization of SU-8 flexible neuroprobe : From mechanical properties to electrophysiological recording. / Huang, Shun Ho; Lin, Shu Ping; Chen, Jia-Jin.

In: Sensors and Actuators, A: Physical, Vol. 216, 01.09.2014, p. 257-265.

Research output: Contribution to journalArticle

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