In vitro assessments of viscoelastic properties of fibrin clot by using acoustic radiation force on a solid sphere

Cho Chiang Shih, Ting Yu Liu, Chih-Chung Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

In present study, the effect of red blood cells concentration on the clot viscoelastic properties was assessed by acoustic radiation force technique in vitro. The experiments were performed on porcine whole blood with different hematocrits from 0 to 40%. Clot formation was induced by adding 2 ml of 0.5 M CaCl2 solution into a 20-ml blood sample. The viscoelastic properties of clot were estimated by detecting the displacement of a solid sphere within clot in response to the applied acoustic radiation force. In experimental system, the solid sphere was pushed by a 1 MHz single element focused transducer. Another single element focused transducer with a center frequency of 20 MHz was used to track the displacements of sphere. For each clot sample, acoustic radiation force tone bursts of 0.25 ms to 7 ms are applied in consecutive experiments. The spatio-temporal behavior of the displacement of sphere was used to assess the viscoelastic properties of clot (with different hematocrits). The experimental result indicates that the value of shear modulus of blood clot decreased from 585±127 Pa at plasma to 168±26 Pa at 40% hematocrit. This result can be used to explain that the concentration of fibrinogen plays the major role in clot elastic properties. In addition, the viscoelastic properties of blood clot can be assessed using a temporal behavior rather than a spatial approach.

Original languageEnglish
Title of host publication2010 IEEE International Ultrasonics Symposium, IUS 2010
Pages479-482
Number of pages4
DOIs
Publication statusPublished - 2010 Dec 1
Event2010 IEEE International Ultrasonics Symposium, IUS 2010 - San Diego, CA, United States
Duration: 2010 Oct 112010 Oct 14

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117

Other

Other2010 IEEE International Ultrasonics Symposium, IUS 2010
CountryUnited States
CitySan Diego, CA
Period10-10-1110-10-14

All Science Journal Classification (ASJC) codes

  • Acoustics and Ultrasonics

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