In vitro diagnostics of coronavirus disease 2019: Technologies and application

Chih Cheng Lai, Cheng Yi Wang, Wen Chien Ko, Po Ren Hsueh

Research output: Contribution to journalReview articlepeer-review

51 Citations (Scopus)


Laboratory-based diagnostic measures including virological and serological tests are essential for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Real-time reverse transcription-polymerase chain reactions (rRT-PCR) can detect SARS-COV-2 by targeting open reading frame-1 antibodies (ORF1ab), envelope protein, nucleocapsid protein, RNA-dependent RNA polymerase genes, and the N1, N2, and N3 (3N) target genes. Therefore, rRT-PCR remains the primary method of diagnosing SARS-CoV-2 despite being limited by false-negative results, long turnaround, complex protocols, and a need for skilled personnel. Serological diagnosis of coronavirus disease 2019 (COVID-19) is simple and does not require complex techniques and equipment, rendering it suitable for rapid detection and massive screening. However, serological tests cannot confirm SARS-CoV-2, and results will be false-negative when antibody concentrations fall below detection limits. Balancing the increased use of laboratory tests, risk of testing errors, need for tests, burden on healthcare systems, benefits of early diagnosis, and risk of unnecessary exposure is a significant and persistent challenge in diagnosing COVID-19.

Original languageEnglish
Pages (from-to)164-174
Number of pages11
JournalJournal of Microbiology, Immunology and Infection
Issue number2
Publication statusPublished - 2021 Apr

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases


Dive into the research topics of 'In vitro diagnostics of coronavirus disease 2019: Technologies and application'. Together they form a unique fingerprint.

Cite this