Abstract
An understanding of collagen ultrastructure is very important for designing biopolymers mimicking collagen functions in tissue engineering, or for diagnosing abnormal collagen structure in clinical study. The present study examined formation of a large population of type I collagen single fibrils under different buffer compositions and temperatures. Fibril structures were investigated by dark-field microscopy and atomic force microscopy (AFM). In the phosphate buffered saline (PBS) buffer, the average lengths of single fibrils were 4.8 ± 2.2, 5.0 ± 1.9 and 9.2 ± 5.0 μm for 37°C, 33°C and 29°C, respectively. The differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. In the sodium phosphate (SP) buffer, the average lengths of single fibrils were 10.6 ± 5.4, 11.1 ± 4.5 and 19.6 ± 11.7 μm for 37°C, 33°C and 29°C, respectively. Similarly, the differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. While at the same temperature, the average lengths of single fibrils differed significantly (P < 0.05) between PBS and SP buffers. Single fibrils formed in SP buffer were found to have greater average length than those formed in PBS buffer.
| Original language | English |
|---|---|
| Pages (from-to) | 413-420 |
| Number of pages | 8 |
| Journal | Bio-Medical Materials and Engineering |
| Volume | 15 |
| Issue number | 6 |
| Publication status | Published - 2005 Dec 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biomaterials
- Biomedical Engineering
Cite this
}
In vitro regulation of single collagen fibril length by buffer compositions and temperature. / Liu, Mine Yine; Yeh, Ming-Long; Luo, Zong Ping.
In: Bio-Medical Materials and Engineering, Vol. 15, No. 6, 01.12.2005, p. 413-420.Research output: Contribution to journal › Article
TY - JOUR
T1 - In vitro regulation of single collagen fibril length by buffer compositions and temperature
AU - Liu, Mine Yine
AU - Yeh, Ming-Long
AU - Luo, Zong Ping
PY - 2005/12/1
Y1 - 2005/12/1
N2 - An understanding of collagen ultrastructure is very important for designing biopolymers mimicking collagen functions in tissue engineering, or for diagnosing abnormal collagen structure in clinical study. The present study examined formation of a large population of type I collagen single fibrils under different buffer compositions and temperatures. Fibril structures were investigated by dark-field microscopy and atomic force microscopy (AFM). In the phosphate buffered saline (PBS) buffer, the average lengths of single fibrils were 4.8 ± 2.2, 5.0 ± 1.9 and 9.2 ± 5.0 μm for 37°C, 33°C and 29°C, respectively. The differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. In the sodium phosphate (SP) buffer, the average lengths of single fibrils were 10.6 ± 5.4, 11.1 ± 4.5 and 19.6 ± 11.7 μm for 37°C, 33°C and 29°C, respectively. Similarly, the differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. While at the same temperature, the average lengths of single fibrils differed significantly (P < 0.05) between PBS and SP buffers. Single fibrils formed in SP buffer were found to have greater average length than those formed in PBS buffer.
AB - An understanding of collagen ultrastructure is very important for designing biopolymers mimicking collagen functions in tissue engineering, or for diagnosing abnormal collagen structure in clinical study. The present study examined formation of a large population of type I collagen single fibrils under different buffer compositions and temperatures. Fibril structures were investigated by dark-field microscopy and atomic force microscopy (AFM). In the phosphate buffered saline (PBS) buffer, the average lengths of single fibrils were 4.8 ± 2.2, 5.0 ± 1.9 and 9.2 ± 5.0 μm for 37°C, 33°C and 29°C, respectively. The differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. In the sodium phosphate (SP) buffer, the average lengths of single fibrils were 10.6 ± 5.4, 11.1 ± 4.5 and 19.6 ± 11.7 μm for 37°C, 33°C and 29°C, respectively. Similarly, the differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. While at the same temperature, the average lengths of single fibrils differed significantly (P < 0.05) between PBS and SP buffers. Single fibrils formed in SP buffer were found to have greater average length than those formed in PBS buffer.
UR - http://www.scopus.com/inward/record.url?scp=32444441794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=32444441794&partnerID=8YFLogxK
M3 - Article
VL - 15
SP - 413
EP - 420
JO - Bio-Medical Materials and Engineering
JF - Bio-Medical Materials and Engineering
SN - 0959-2989
IS - 6
ER -