In vitro regulation of single collagen fibril length by buffer compositions and temperature

Mine Yine Liu, Ming-Long Yeh, Zong Ping Luo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An understanding of collagen ultrastructure is very important for designing biopolymers mimicking collagen functions in tissue engineering, or for diagnosing abnormal collagen structure in clinical study. The present study examined formation of a large population of type I collagen single fibrils under different buffer compositions and temperatures. Fibril structures were investigated by dark-field microscopy and atomic force microscopy (AFM). In the phosphate buffered saline (PBS) buffer, the average lengths of single fibrils were 4.8 ± 2.2, 5.0 ± 1.9 and 9.2 ± 5.0 μm for 37°C, 33°C and 29°C, respectively. The differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. In the sodium phosphate (SP) buffer, the average lengths of single fibrils were 10.6 ± 5.4, 11.1 ± 4.5 and 19.6 ± 11.7 μm for 37°C, 33°C and 29°C, respectively. Similarly, the differences were significant (P < 0.05) between 37°C and 29°C and between 33°C and 29°C. While at the same temperature, the average lengths of single fibrils differed significantly (P < 0.05) between PBS and SP buffers. Single fibrils formed in SP buffer were found to have greater average length than those formed in PBS buffer.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalBio-Medical Materials and Engineering
Volume15
Issue number6
Publication statusPublished - 2005 Dec 1

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Collagen
Buffers
Phosphates
Chemical analysis
Sodium
Temperature
Biopolymers
Collagen Type I
Tissue engineering
Atomic force microscopy
Microscopic examination
sodium phosphate

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

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In vitro regulation of single collagen fibril length by buffer compositions and temperature. / Liu, Mine Yine; Yeh, Ming-Long; Luo, Zong Ping.

In: Bio-Medical Materials and Engineering, Vol. 15, No. 6, 01.12.2005, p. 413-420.

Research output: Contribution to journalArticle

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