TY - JOUR
T1 - In vivo determination of skin near-infrared optical properties using diffuse optical spectroscopy
AU - Tseng, Sheng Hao
AU - Grant, Alexander
AU - Durkin, Anthony J.
N1 - Funding Information:
This work was supported by the National Institutes of Health/ National Center for Research Resources (NCRR) under Grant No. P41-RR01192 (Laser Microbeam and Medical Program: LAMMP), the U.S. Air Force Office of Scientific Research, the Medical Free-Electron Laser Program (F49620-00-2-0371 and FA9550-04-1-0101), and the Beckman Foundation.
PY - 2008
Y1 - 2008
N2 - We develop a superficial diffusing probe with a 3mm source-detector separation that can be used in combination with diffuse optical spectroscopic (DOS) methods to noninvasively determine full-spectrum optical properties of superficial in vivo skin in the wavelength range from 650 to 1000nm. This new probe uses a highly scattering layer to diffuse photons emitted from a collimated light source and relies on a two-layer diffusion model to determine tissue absorption coefficient μ a and reduced scattering coefficient μ s ′. By employing the probe to measure two-layer phantoms that mimic the optical properties of skin, we demonstrate that the probe has an interrogation depth of 1 to 2mm. We carry out SSFDPM (steady state frequency-domain photon migration) measurements using this new probe on the volar forearm and palm of 15 subjects, including five subjects of African descent, five Asians, and five Caucasians. The optical properties of in vivo skin determined using the superficial diffusing probe show considerable similarity to published optical properties of carefully prepared ex vivo epidermis+dermis.
AB - We develop a superficial diffusing probe with a 3mm source-detector separation that can be used in combination with diffuse optical spectroscopic (DOS) methods to noninvasively determine full-spectrum optical properties of superficial in vivo skin in the wavelength range from 650 to 1000nm. This new probe uses a highly scattering layer to diffuse photons emitted from a collimated light source and relies on a two-layer diffusion model to determine tissue absorption coefficient μ a and reduced scattering coefficient μ s ′. By employing the probe to measure two-layer phantoms that mimic the optical properties of skin, we demonstrate that the probe has an interrogation depth of 1 to 2mm. We carry out SSFDPM (steady state frequency-domain photon migration) measurements using this new probe on the volar forearm and palm of 15 subjects, including five subjects of African descent, five Asians, and five Caucasians. The optical properties of in vivo skin determined using the superficial diffusing probe show considerable similarity to published optical properties of carefully prepared ex vivo epidermis+dermis.
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U2 - 10.1117/1.2829772
DO - 10.1117/1.2829772
M3 - Article
C2 - 18315374
AN - SCOPUS:44849095838
SN - 1083-3668
VL - 13
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
IS - 1
M1 - 014016
ER -